Influence of resuscitation volume on blood cells TNF production in a murine model of haemorrhage

The influence of haemorrhage and resuscitation on Tumour Necrosis Factor (TNF) production by whole blood cultures under endotoxin (Escherichia coli LPS) stimulation was investigated in mate BALB/c mice. Haemorrhagic shock was induced by removing 0.026 +/- 0.003 mL of blood/g via a cardiac puncture, resulting in a 50% decrease in arterial pressure and a metabolic adidosis. Animals were resuscitated successfully (normotensive) despite a residual base deficit and hyperlactatemia, 60 min after the haemorrhage by the restitution of shed blood volume (SBV) with or without an additional volume of crystalloid (Lactated Ringer's solution) equal to 50, 100 (isovotumetric resuscitation) or 200% of SBV. Pulmonary failure (hypoxiahypercarbia) and myocardial injury (troponin I release) was observed in this last group. TNF production by whole blood cultures stimulated ex vivo by LIPS was estimated 60 min after the end of resuscitation. Haemorrhage resulted in a 48-60% decrease in TNF production. This decrease so-called 'leukocyte deactivation' was not modified by the restitution of SBV with or without crystalloid except for isovolunnetric resuscitation which resulted in the cytokine level. returning to control in the absence of clear cardiopulmonary dysfunction. In the present murine model. of haemorrhage, modifying resuscitation volume influences in vitro TNF production in whole blood cultures challenged by LPS. (c) 2005 Elsevier Ireland Ltd. All rights reserved.