Conservation patterns in angiosperm rDNA ITS2 sequences

被引:147
作者
Hershkovitz, MA [1 ]
Zimmer, EA [1 ]
机构
[1] SMITHSONIAN INST,LAB MOL SYSTEMAT,WASHINGTON,DC 20560
关键词
D O I
10.1093/nar/24.15.2857
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The two internal transcribed spacers (ITS1 and ITS2) of nuclear ribosomal DNA have become commonly exploited sources of informative variation for interspecific-/intergeneric-level phylogenetic analyses among angiosperms and other eukaryotes, We present an alignment in which one-third to one-half of the ITS2 sequence is alignable above the family level in angiosperms and a phenetic analysis showing that ITS2 contains information sufficient to diagnose lineages at several hierarchical levels. Base compositional analysis shows that angiosperm ITS2 is inherently CC-rich, and that the proportion of T is much more variable than that for other bases, We propose a general model of angiosperm ITS2 secondary structure that shows common pairing relationships for most of the conserved sequence tracts. Variations in our secondary structure predictions for sequences from different taxa indicate that compensatory mutation is not limited to paired positions.
引用
收藏
页码:2857 / 2867
页数:11
相关论文
共 49 条
[1]   EVOLUTION OF NUCLEAR RDNA ITS SEQUENCES IN THE CLADOPHORA ALBIDA/SERICEA CLADE (CHLOROPHYTA) [J].
BAKKER, FT ;
OLSEN, JL ;
STAM, WT .
JOURNAL OF MOLECULAR EVOLUTION, 1995, 40 (06) :640-651
[2]   THE ITS REGION OF NUCLEAR RIBOSOMAL DNA - A VALUABLE SOURCE OF EVIDENCE ON ANGIOSPERM PHYLOGENY [J].
BALDWIN, BG ;
SANDERSON, MJ ;
PORTER, JM ;
WOJCIECHOWSKI, MF ;
CAMPBELL, CS ;
DONOGHUE, MJ .
ANNALS OF THE MISSOURI BOTANICAL GARDEN, 1995, 82 (02) :247-277
[3]   The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny (vol 82, pg 247, 1995) [J].
Baldwin, BG ;
Sanderson, MJ ;
Porter, JM ;
Wojciechowski, MF ;
Campbell, CS ;
Donoghue, MJ .
ANNALS OF THE MISSOURI BOTANICAL GARDEN, 1996, 83 (01) :151-151
[4]   Phylogenetic Utility of the Internal Transcribed Spacers of Nuclear Ribosomal DNA in Plants: An Example from the Compositae [J].
Baldwin, Bruce G. .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 1992, 1 (01) :3-16
[5]  
Bremer K., 1994, ASTERACEAE CLADISTIC
[6]  
Campbell B.C., 1993, Insect Molecular Biology, V2, P225
[7]  
CHASE M. W., 1995, Monocotyledons: systematics and evolution, V1, P685
[8]   PHYLOGENETICS OF SEED PLANTS - AN ANALYSIS OF NUCLEOTIDE-SEQUENCES FROM THE PLASTID GENE RBCL [J].
CHASE, MW ;
SOLTIS, DE ;
OLMSTEAD, RG ;
MORGAN, D ;
LES, DH ;
MISHLER, BD ;
DUVALL, MR ;
PRICE, RA ;
HILLS, HG ;
QIU, YL ;
KRON, KA ;
RETTIG, JH ;
CONTI, E ;
PALMER, JD ;
MANHART, JR ;
SYTSMA, KJ ;
MICHAELS, HJ ;
KRESS, WJ ;
KAROL, KG ;
CLARK, WD ;
HEDREN, M ;
GAUT, BS ;
JANSEN, RK ;
KIM, KJ ;
WIMPEE, CF ;
SMITH, JF ;
FURNIER, GR ;
STRAUSS, SH ;
XIANG, QY ;
PLUNKETT, GM ;
SOLTIS, PS ;
SWENSEN, SM ;
WILLIAMS, SE ;
GADEK, PA ;
QUINN, CJ ;
EGUIARTE, LE ;
GOLENBERG, E ;
LEARN, GH ;
GRAHAM, SW ;
BARRETT, SCH ;
DAYANANDAN, S ;
ALBERT, VA .
ANNALS OF THE MISSOURI BOTANICAL GARDEN, 1993, 80 (03) :528-580
[9]  
Chase MW., 1995, MONOCOTYLEDONS SYSTE, V1, P109, DOI DOI 10.1016/0378-8741(87)90103-6
[10]  
CMAPBELL CS, 1995, AM J BOT, V82, P903