13C{13C} 2D NMR:: A novel strategy for the study of paramagnetic proteins with slow electronic relaxation rates

被引：74

作者：

Machonkin, TE ^{[1
]}

Westler, WM ^{[1
]}

Markley, JL ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biochem, Natl Magnet Resonance Facil Madison, Madison, WI 53706 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 13期

关键词：

D O I：

10.1021/ja017733j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oxidized human [2Fe-2S] ferredoxin has a notably slow electronic relaxation rate, which precludes the observation of signals from nuclei near the iron-sulfur cluster by conventional 2D or 3D methods that utilize proton detection. We have demonstrated the utility of 13C{13C} CT-COSY in identifying connectivity information from fast relaxing carbon nuclei near the paramagnetic center, including those from residues that ligate the metal center. Copyright © 2002 American Chemical Society.

引用

页码：3204 / 3205

页数：2

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