The genome of M-acetivorans reveals extensive metabolic and physiological diversity

被引:485
作者
Galagan, JE
Nusbaum, C
Roy, A
Endrizzi, MG
Macdonald, P
FitzHugh, W
Calvo, S
Engels, R
Smirnov, S
Atnoor, D
Brown, A
Allen, N
Naylor, J
Stange-Thomann, N
DeArellano, K
Johnson, R
Linton, L
McEwan, P
McKernan, K
Talamas, J
Tirrell, A
Ye, WJ
Zimmer, A
Barber, RD
Cann, I
Graham, DE
Grahame, DA
Guss, AM
Hedderich, R
Ingram-Smith, C
Kuettner, HC
Krzycki, JA
Leigh, JA
Li, WX
Liu, JF
Mukhopadhyay, B
Reeve, JN
Smith, K
Springer, TA
Umayam, LA
White, O
White, RH
de Macario, EC
Ferry, JG
Jarrell, KF
Jing, H
Macario, AJL
Paulsen, I
Pritchett, M
Sowers, KR
机构
[1] Whitehead Inst Ctr Genome Res, Cambridge, MA 02141 USA
[2] Univ Wisconsin Parkside, Dept Biol Sci, Kenosha, WI 53141 USA
[3] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA
[4] Virginia Polytech Inst & State Univ, Dept Biochem, Blacksburg, VA 24061 USA
[5] Uniformed Serv Univ Hlth Sci, Dept Biochem & Mol Biol, Bethesda, MD 20814 USA
[6] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA
[7] Max Planck Inst Terr Mikrobiol, D-35043 Marburg, Germany
[8] Clemson Univ, Dept Biochem & Genet, Clemson, SC 29634 USA
[9] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
[10] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[11] Univ Kentucky, TH Morgan Sch Biol Sci, Lexington, KY 40506 USA
[12] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA
[13] Harvard Univ, Sch Med, Ctr Blood Res, Boston, MA 02115 USA
[14] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA
[15] Inst Genomic Res, Rockville, MD 20878 USA
[16] SUNY Albany, Dept Biomed Sci, Sch Publ Hlth, Albany, NY 12201 USA
[17] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA
[18] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
[19] Queens Univ, Dept Microbiol & Immunol, Kingston, ON KYL 3N6, Canada
[20] Univ Maryland, Ctr Marine Biotechnol, Inst Biotechnol, Baltimore, MD 21202 USA
[21] Syrrx Inc, San Diego, CA 92121 USA
[22] Cornell Univ, Ithaca, NY 14853 USA
[23] MIT, Dept Biol, Cambridge, MA 02139 USA
关键词
D O I
10.1101/gr.223902
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived from acetate. Here we report the complete genome sequence of an acetate-utilizing methanogen, Methanosarcina acetivorans C2A. Methanosarcineae are the most metabolically diverse methanogens, thrive in a broad range of environments, and are unique among the Archaea in forming complex multicellular structures. This diversity Is reflected In the genome of M. acetivorans. At 5,751,492 base pairs it is by far the largest known archaeal genome. The 4524 open reading frames code for a strikingly wide and unanticipated variety of metabolic and cellular capabilities. The presence of novel methyltransferases indicates the likelihood of undiscovered natural energy sources for methanogenesis, whereas the presence of single-subunit carbon monoxide dehydrogenases raises the possibility of nonmethanogenic growth. Although motility has not been observed in any Methanosarcineae, a flagellin gene cluster and two complete chemotaxis gene clusters were identified. The availability of genetic methods, coupled with its physiological and metabolic diversity, makes M. acetivorans a powerful model organism for the study of archaeal biology.
引用
收藏
页码:532 / 542
页数:11
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