Enhancement of tolerance to heavy metals and oxidative stress in Dunaliella tertiolecta by Zn-induced phytochelatin synthesis

被引:113
作者
Tsuji, N
Hirayanagi, N
Okada, M
Miyasaka, H
Hirata, K
Zenk, MH
Miyamoto, K
机构
[1] Osaka Univ, Grad Sch Pharmaceut Sci, Environm Bioengn Lab, Suita, Osaka 5650871, Japan
[2] Kansai Elect Power Co, Tech Res Ctr, Amagasaki, Hyogo 6710974, Japan
[3] Univ Halle Wittenberg, Biozentrum Pharmazie, D-06120 Halle An Der Saale, Germany
关键词
phytochelatins; zinc; heavy metals; oxidative stress;
D O I
10.1016/S0006-291X(02)00265-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The synthesis of phytochelatins (PCs) in a marine alga, Dunalliela tertiolecta, is strongly induced by Zn. Pretreatment of the cells with Zn enhances the tolerance toward toxic heavy metals such as Cd, Hg. Cu, Pb, and arsenate. Moreover, the pretreatment also increases the tolerance toward oxidative stress caused by hydrogen peroxide or paraquat. In vitro analysis shows that PC is a stronger scavenger of hydrogen peroxide and superoxide radical than glutathione. These results Suggest that PCs inducibly synthesized by Zn treatment could play a role not only in detoxification of heavy metals but also in mitigation of oxidative stress. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:653 / 659
页数:7
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