Muscle growth and muscle function: A molecular biological perspective

Molecular biological methods are pervading all biomedical fields and it is likely that they will soon introduce new techniques to veterinary diagnostics and have a major impact on food and fibre production in animal agriculture. The ability to manipulate muscle growth and phenotype will present new ethical problems, particularly if the techniques are used to manipulate muscle development in greyhounds and racehorses where the financial rewards could be very substantial. Muscle has been a useful tissue for the study of the molecular control of tissue development because terminal differentiation results in the production of large quantities of highly specialised proteins. Now that the functional anatomy of structural genes in muscle is being elucidated, a coherent picture is beginning to emerge of the way in which post-natal muscle growth and phenotype are regulated at the gene level. The hormones and growth factors involved in regulating the quantitative and qualitative changes in gene expression are now better understood, together with the ability of the tissue to adapt to physical signals and hence new activity patterns. The myosin heavy chain isoform genes which encode the myosin cross-bridges (the force generators for muscular contraction) exist as a large multigene family. The contractility and other characteristics of muscle depend to a large extent on the differential expression of members of this and other gene families. Muscle fibres adapt for increased power output by expressing a subset of 'fast' genes and for increased economy of action by expressing a slow subset of genes and producing more mitochondria. With the increasing understanding of gene expression in muscle, there are prospects for manipulating the mass, contractility and other characteristics of muscle and also to change its phenotype and understand certain disease states.