Collagen from common minke whale (Balaenoptera acutorostrata) unesu

被引：124

作者：

Nagai, Takeshi ^{[1
]}

Suzuki, Nobutaka ^{[2
]}

Nagashima, Toshio ^{[1
]}

机构：

[1] Tokyo Univ Agr, Dept Food Sci & Technol, Abashiri, Hokkaido 0992493, Japan

[2] Nagoya Res Inst, Aichi 4701131, Japan

来源：

FOOD CHEMISTRY | 2008年 / 111卷 / 02期

关键词：

collagen; common minke whale unesu; attenuated total reflectance-Fourier; transform infra-red (ATR-FTIR) analysis;

D O I：

10.1016/j.foodchem.2008.03.087

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Collagen was prepared from common minke whale unesu and characterised. The yield of collagen was high, about 28.4% on a wet weight basis. By SDS-PAGE and CM-Toyopearl 650 M column chromatography, the collagen was classified as type I collagen. The denaturation temperature of the collagen was 31.5 degrees C, about 6-7 degrees C lower than that of porcine collagen. Attenuated total reflectance-FTIR analysis indicated that acid-soluble collagen from common minke whale unesu held its triple helical structure well, but the structures of porcine skin collagen and pepsin-solubilized collagen from common minke whale unesu were changed slightly, due to the loss of N- and C-terminus domains. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：296 / 301

页数：6

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