Electrochemical oxidation of cholesterol catalyzed by cholesterol oxidase with use of an artificial electron mediator

被引:67
作者
Nakaminami, T [1 ]
Kuwabata, S [1 ]
Yoneyama, H [1 ]
机构
[1] OSAKA UNIV,FAC ENGN,DEPT APPL CHEM,SUITA,OSAKA 565,JAPAN
关键词
D O I
10.1021/ac960996p
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Several redox dyes of phenazine and phenothiazine derivatives including 1-methoxy-5-methylphenazinium methosulfate (MPMS), thionin, azure A, and azure C work well as electron accepters for reduced cholesterol oxidase (EC 1.1.3.6) (ChOx). Kinetic parameters and equilibrium constants of the enzymatic oxidation of cholesterol obtained suggest that the molecular size and/or hydrophobicity of the redox dyes determines their abilities as the electron accepters. The electrochemical sensitivity to cholesterol in the detection system using ChOx and the above-mentioned redox compounds is determined by the electron acceptability of the redox compounds, and the employment of either MPMS or thionin allows electrochemical determination of cholesterol at a low electrode potential (0 V vs SCE) in the concentration range 0.25-2.5 mmol dm(-3), which covers that in normal human blood.
引用
收藏
页码:2367 / 2372
页数:6
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