Acceptor specificity of GDP-Fuc:Gal beta 1->4GlcNAc-R alpha 3-fucosyltransferase VI (FucT VI) expressed in insect cells as soluble, secreted enzyme

被引：49

作者：

DeVries, T ^{[1
]}

Palcic, MP ^{[1
]}

Schoenmakers, PS ^{[1
]}

VandenEijnden, DH ^{[1
]}

Joziasse, DH ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON,AB T6G 2G2,CANADA

来源：

GLYCOBIOLOGY | 1997年 / 7卷 / 07期

基金：

英国医学研究理事会;

关键词：

baculovirus; enzyme-assisted synthesis; glycosyltransferase; Lewis x;

D O I：

10.1093/glycob/7.7.921

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

As an extension of a previous study (de Vries et nl,, 1995, J, Biol. Chern., 270, 8712-8722) the acceptor specificity of recombinant FucT VI, expressed in insect cells as soluble enzyme, and purified from the growth medium by affinity chromatography, was analyzed toward a broad panel of oligosaccharide and glycoprotein substrates. It was found that FucT VI effectively utilizes any type-2-chain based structure (Gal beta 1-->4GlcNAc-R), Neutral as well as sialylated structures are fucosylated with high efficiency, To identify polar groups on accepters that function in enzyme binding, deoxygenated substrate analogs were tested as accepters, FucT VI had an absolute requirement for a hydroxyl at C-6 of galactose in addition to the accepting hydroxyl at C-3, Thus, FucT VI, although different from FucT III, TV, and V in acceptor properties, seems to bind the acceptor in a similar way.

引用

页码：921 / 927

页数：7

共 40 条

[1]

BEYER TA, 1980, J BIOL CHEM, V255, P5364

[2]

A missense mutation in the FUT6 gene results in total absence of alpha 3-fucosylation of human alpha(1)-acid glycoprotein [J].