Thyrotropin-releasing activity of histone H2A, H2B and peptide MB35

被引：7

作者：

Brown, OA

Sosa, YE

Goya, RG

机构：

[1] NATL UNIV LA PLATA,FAC MED,INIBIOLP,RA-1900 LA PLATA,ARGENTINA

[2] NATL UNIV LA PLATA,SCH MED,DEPT HISTOL B,RA-1900 LA PLATA,ARGENTINA

来源：

PEPTIDES | 1997年 / 18卷 / 09期

基金：

英国惠康基金;

关键词：

histones; MB35; pituitary cells; TSH release; calcium; diacylglycerol;

D O I：

10.1016/S0196-9781(97)00204-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histones possess multiple hormone-like activities. We studied the specificity and signal transduction pathways involved in the thyrotrophin (TSH)-releasing activity of histones H2A, H2B and peptide MB35, a H2A fragment, using perifused and incubated dispersed rat pituitary cells and measuring TSH release by a specific R1A. Histones released TSH in a dose- and time-dependent fashion while peptide MB35 behaved as a weaker secretagogue. Trifluoperazine and EGTA blocked histone-stimulated TSH release while forskolin and other cAMP enhancers did not. We conclude that the TSH-releasing activity of histones H2A and H2B is mediated by calcium-and diacylglycerol-associated pathways. (C) 1997 Elsevier Science Inc.

引用

页码：1315 / 1319

页数：5

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