Interaction between prostanoids and nitric oxide in the control of tubular function in rats with chronic bile duct ligation

被引:13
作者
Criado, M
Flores, O
Hidalgo, F
López-Novoa, JM
Sánchez-Rodríguez, A
机构
[1] Univ Salamanca, Dept Fisiol & Farmacol, Inst Reine Sofia Invest Nefrol, Edificio Dept, Salamanca 37007, Spain
[2] Univ Salamanca, Dept Med, Salamanca 37007, Spain
关键词
kidney; biliary cirrhosis; nitric oxide synthase; cyclooxygenase;
D O I
10.1139/cjpp-77-2-111
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Recent work indicates that both nitric oxide and cyclooxygenase products play an important role in the renal alterations of liver cirrhosis, although the interactions between them have not been completely established. The purpose of this study was to assess the effect of simultaneous blockade of nitric oxide synthase and cyclooxygenase in rats with chronic bile duct ligation and in control, sham-operated rats. Compared with control rats, chronic bile duct ligation rats, 23-25 days after surgery, showed a decreased mean arterial pressure, natriuresis, and kaliuresis, without differences in glomerular filtration rate, and an increased urinary nitrite excretion. Nitric oxide synthesis inhibition by administration of N-G-nitro-L-arginine methyl ester induced, in control rats, an increase in mean arterial pressure, without significant changes in natriuresis or glomerular filtration rate. In chronic bile duct ligation rats, N-G-nitro-L-arginine methyl ester induced an increase in mean arterial pressure, natriuresis, and kaliuresis, together with a reduction in urinary nitrite excretion and an increase in prostaglandin E-2 excretion. Cyclooxygenase inhibition with indomethacin induced in both experimental groups a marked inhibition in urinary prostaglandin E-2 excretion without significant changes in Na+ or K+ excretion, and a significant increase in urinary nitrite excretion in control rats. N-G-Nitro-L-arginine methyl ester in addition to indomethacin prevented the indomethacin-induced increase in nitrite excretion and dramatically reduced sodium excretion in both experimental groups. Thus, the present study suggests that both nitric oxide and cyclooxygenase products interact in the control of urinary sodium excretion and that each system is activated in the absence of the other one.
引用
收藏
页码:111 / 117
页数:7
相关论文
共 33 条
[1]   RENAL EFFECTS OF NITRIC-OXIDE SYNTHESIS INHIBITION IN CIRRHOTIC RATS [J].
ATUCHA, NM ;
GARCIAESTAN, J ;
RAMIREZ, A ;
PEREZ, MD ;
QUESADA, T ;
ROMERO, JC .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1994, 267 (06) :R1454-R1460
[2]   MICROPUNCTURE STUDY OF RENAL SALT AND WATER RETENTION IN CHRONIC BILE-DUCT OBSTRUCTION [J].
BANK, N ;
AYNEDJIAN, HS .
JOURNAL OF CLINICAL INVESTIGATION, 1975, 55 (05) :994-1002
[3]   CHRONIC BLOCKADE OF NITRIC-OXIDE SYNTHESIS IN THE RAT PRODUCES SYSTEMIC HYPERTENSION AND GLOMERULAR DAMAGE [J].
BAYLIS, C ;
MITRUKA, B ;
DENG, A .
JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (01) :278-281
[4]  
BETTER OS, 1988, KIDNEY LIVER DIS, P508
[5]  
BOLGER PM, 1978, NATURE, V271, P467
[6]  
BONSNES RW, 1945, J BIOL CHEM, V158, P581
[7]  
BOSCH J, 1988, KDINEY LIVER DIS, P268
[8]  
Bretaudiere J. P., 1984, METHOD ENZYMAT AN, P75
[9]   GLOMERULI SYNTHESIZE NITRITE IN EXPERIMENTAL NEPHROTOXIC NEPHRITIS [J].
CATTELL, V ;
COOK, T ;
MONCADA, S .
KIDNEY INTERNATIONAL, 1990, 38 (06) :1056-1060
[10]   PATHOGENESIS OF ARTERIAL-HYPOTENSION IN CIRRHOTIC RATS WITH ASCITES - ROLE OF ENDOGENOUS NITRIC-OXIDE [J].
CLARIA, J ;
JIMENEZ, W ;
ROS, J ;
ASBERT, M ;
CASTRO, A ;
ARROYO, V ;
RIVERA, F ;
RODES, J .
HEPATOLOGY, 1992, 15 (02) :343-349