Mechanisms of dCMP transferase reactions catalyzed by mouse Rev1 protein

被引:44
作者
Masuda, Y [1 ]
Takahashi, M [1 ]
Fukuda, S [1 ]
Sumii, M [1 ]
Kamiya, K [1 ]
机构
[1] Hiroshima Univ, Res Inst Radiat Biol & Med, Minami Ku, Hiroshima 7348553, Japan
关键词
D O I
10.1074/jbc.M110149200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Rev1 protein, a member of a large family of translesion DNA polymerases, catalyzes a dCMP transfer reaction. Recombinant mouse Rev1 protein was found to insert a dCMP residue opposite guanine, adenine, thymine, cytosine, uracil, and an apurinic/apyrimidinic site and to have weak ability for transfer to a mismatched terminus. The mismatch-extension ability was strongly enhanced by a guanine residue on the template near the mismatched terminus; this was not the case with an apurinic/apyrimidinic site and the other template nucleotides. Kinetic analysis of the dCMP transferase reaction provided evidence for high affinity for dCTP with template G but not the other templates, whereas the template nucleotide did not much affect the V-max value. Furthermore, it could be established that the mouse Rev1 protein inserts dGMP and dTMP residues opposite template guanine at a V-max similar to that for dCMP.
引用
收藏
页码:3040 / 3046
页数:7
相关论文
共 27 条
[1]   Distinct roles for Rev1p and Rev7p during translesion synthesis in Saccharomyces cerevisiae [J].
Baynton, K ;
Bresson-Roy, A ;
Fuchs, RPP .
MOLECULAR MICROBIOLOGY, 1999, 34 (01) :124-133
[2]   From BRCA1 to RAP1: A widespread BRCT module closely associated with DNA repair [J].
Callebaut, I ;
Mornon, JP .
FEBS LETTERS, 1997, 400 (01) :25-30
[3]   Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway [J].
Desany, BA ;
Alcasabas, AA ;
Bachant, JB ;
Elledge, SJ .
GENES & DEVELOPMENT, 1998, 12 (18) :2956-2970
[4]   RIBONUCLEOTIDE REDUCTASE - REGULATION, REGULATION, REGULATION [J].
ELLEDGE, SJ ;
ZHENG, Z ;
ALLEN, JB .
TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (03) :119-123
[5]   NOVEL MUTAGENIC PROPERTIES OF ABASIC SITES IN SACCHAROMYCES-CEREVISIAE [J].
GIBBS, PEM ;
LAWRENCE, CW .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 251 (02) :229-236
[6]   The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light [J].
Gibbs, PEM ;
Wang, XD ;
Li, ZQ ;
McManus, TP ;
McGregor, WG ;
Lawrence, CW ;
Maher, VM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (08) :4186-4191
[7]   Generation of a strong mutator phenotype in yeast by imbalanced base excision repair [J].
Glassner, BJ ;
Rasmussen, LJ ;
Najarian, MT ;
Posnick, LM ;
Samson, LD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :9997-10002
[8]   Roles of yeast DNA polymerases δ and ζ and of Rev1 in the bypass of abasic sites [J].
Haracska, L ;
Unk, I ;
Johnson, RE ;
Johansson, E ;
Burgers, PMJ ;
Prakash, S ;
Prakash, L .
GENES & DEVELOPMENT, 2001, 15 (08) :945-954
[9]   DNA polymerase ζ introduces multiple mutations when bypassing spontaneous DNA damage in Saccharomyces cerevisiae [J].
Harfe, BD ;
Jinks-Robertson, S .
MOLECULAR CELL, 2000, 6 (06) :1491-1499
[10]   ANALYSIS OF SPONTANEOUS FRAMESHIFT MUTATIONS IN REV1 AND REV1-1 STRAINS OF SACCHAROMYCES-CEREVISIAE [J].
KALINOWSKI, DP ;
LARIMER, FW ;
PLEWA, MJ .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1995, 331 (01) :149-159