No effect of recombinase-mediated DNA transfer on production efficiency of transgenic rats

被引：4

作者：

Hirabayashi, M ^{[1
]}

Kato, M

Kaneko, R

Hirabayashi, T

Morita, M

Hochi, S

机构：

[1] Natl Inst Physiol Sci, Okazaki, Aichi 4448787, Japan

[2] Grad Univ Adv Studies, Okazaki, Aichi 4448787, Japan

[3] Tokyo Univ Pharm & Life Sci, Hachioji, Tokyo 1920392, Japan

[4] Shinshu Univ, Fac Text Sci & Technol, Ueda, Nagano 3868567, Japan

来源：

EXPERIMENTAL ANIMALS | 2006年 / 55卷 / 02期

关键词：

rat; RecA; transgenesis;

D O I：

10.1538/expanim.55.131

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

It was reported that recombinase-A protein (RecA)-coated exogenous DNA was more likely to be integrated into mouse, goat and pig genomes. The objective of this study was to investigate whether integration of exogenous DNA into the rat genome is improved by the recombinase-mediated DNA transfer. Pronuclear microinjection of RecA-coated EGFP or OAMB DNA resulted in a production efficiency of transgenic rats of 1.4-2.9%, comparable with 0.9-2.6% when non-coated control DNA was used. Intracytoplasmic injection of the sperm heads exposed to RecA-coated EGFP DNA did not produce any transgenic rats (0 vs. 0-2.8% in control groups). Thus, the recombinase-mediated DNA transfer contributed very little to the production of transgenic rats by means of pronuclear microinjection and intracytoplasmic sperm injection.

引用

页码：131 / 135

页数：5

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