Purification and cloning of aggrecanase-1: A member of the ADAMTS family of proteins

被引：571

作者：

Tortorella, MD

Burn, TC

Pratta, MA

Abbaszade, I

Hollis, JM

Liu, R

Rosenfeld, SA

Copeland, RA

Decicco, CP

Wynn, R

Rockwell, A

Yang, F

Duke, JL

Solomon, K

George, H

Bruckner, R

Nagase, H

Itoh, Y

Ellis, DM

Ross, H

Wiswall, BH

Murphy, K

Hillman, MC

Hollis, GF

Newton, RC

Magolda, RL

Trzaskos, JM

Arner, EC ^{[1
]}

机构：

[1] Dupont Merck Pharmaceut Co, Dept Inflammatory Dis Res, Wilmington, DE 19880 USA

[2] Dupont Merck Pharmaceut Co, Dept Appl Biotechnol, Wilmington, DE 19880 USA

[3] Dupont Merck Pharmaceut Co, Dept Enzymol, Wilmington, DE 19880 USA

[4] Dupont Merck Pharmaceut Co, Dept Chem & Phys Sci, Wilmington, DE 19880 USA

[5] Univ Kansas, Sch Med, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA

来源：

SCIENCE | 1999年 / 284卷 / 5420期

关键词：

D O I：

10.1126/science.284.5420.1664

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We purified,cloned, and expressed aggrecanase, a protease that is thought to be responsible for the:degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4)] is a member of the: ADAMTS protein family that cleaves aggrecan at the glutamic acid-373-alanine-374 bond. The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.

引用

页码：1664 / 1666

页数：3

共 31 条

[1] Cytokine-induced cartilage proteoglycan degradation is mediated by aggrecanase
Arner, EC
Hughes, CE
Decicco, CP
Caterson, B
Tortorella, MD
[J]. OSTEOARTHRITIS AND CARTILAGE, 1998, 6 (03) : 214 - 228
[2] Generation and characterization of aggrecanase - A soluble, cartilage-derived aggrecan-degrading activity
Arner, EC
Pratta, MA
Trzaskos, JM
Decicco, CP
Tortorella, MD
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (10) : 6594 - 6601
[3] Isothiazolones interfere with normal matrix metalloproteinase activation and inhibit cartilage proteoglycan degradation
Arner, EC
Pratta, MA
Freimark, B
Lischwe, M
Trzaskos, JM
Magolda, RL
Wright, SW
[J]. BIOCHEMICAL JOURNAL, 1996, 318 : 417 - 424
[4] ARNER EC, UNPUB
[5] MAMMALIAN SUBTILISINS - THE LONG-SOUGHT DIBASIC PROCESSING ENDOPROTEASES
BARR, PJ
[J]. CELL, 1991, 66 (01) : 1 - 3
[6] CHARACTERIZATION AND USE OF THE DROSOPHILA METALLOTHIONEIN PROMOTER IN CULTURED DROSOPHILA-MELANOGASTER CELLS
BUNCH, TA
GRINBLAT, Y
GOLDSTEIN, LSB
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (03) : 1043 - 1061
[7] BURN TC, IN PRESS J BIOL CHEM
[8] LOCALIZATION OF BETA-1-INTEGRINS IN HUMAN CARTILAGE AND THEIR ROLE IN CHONDROCYTE ADHESION TO COLLAGEN AND FIBRONECTIN
DURR, J
GOODMAN, S
POTOCNIK, A
VONDERMARK, H
VONDERMARK, K
[J]. EXPERIMENTAL CELL RESEARCH, 1993, 207 (02) : 235 - 244
[9] FLANNERY CR, 1992, J BIOL CHEM, V267, P1008
[10] FOSANG AJ, 1991, J BIOL CHEM, V266, P15579

← 1 2 3 4 →