Synthesis of α-galacto-oligosaccharides by a cloned α-galactosidase from Bifidobacterium adolescentis

被引：37

作者：

van den Broek, LAM

Ton, J

Verdoes, JC

Van Laere, KMJ

Voragen, AGJ

Beldman, G

机构：

[1] Agr Univ Wageningen, Dept Food Technol & Nutr Sci, Div Food Sci, Food Chem Chair, NL-6700 EV Wageningen, Netherlands

[2] Agr Univ Wageningen, Dept Food Technol & Nutr Sci, Ind Microbiol Chair, NL-6700 EV Wageningen, Netherlands

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 05期

关键词：

Bifidobacterium adolescentis; alpha-galacto-oligosaccharides; alpha-galactosidase; prebiotics; transglycosylation;

D O I：

10.1023/A:1005542521708

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A genomic library of Bifidobacterium adolescentis was constructed in Escherichia coli and a gene encoding an alpha-galactosidase was isolated. The identified open reading frame showed high similarity and identity with bacterial alpha-galactosidases, which belong to Family 36 of the glycosyl hydrolases. For the purification of the enzyme from the medium a single chromatography step was sufficient. The yield of the recombinant enzyme was 100 times higher than from B. adolescentis itself. In addition to hydrolytic activity the alpha-galactosidase showed transglycosylation activity and can be used for the production of alpha-galacto-oligosaccharides.

引用

页码：441 / 445

页数：5

共 18 条

[1] BIOCHEMICAL AND GENETIC-ANALYSIS OF STREPTOCOCCUS-MUTANS ALPHA-GALACTOSIDASE [J].

ADUSEOPOKU, J ;

TAO, L ;

FERRETTI, JJ ;

RUSSELL, RRB .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :757-764

[2] NUCLEOTIDE-SEQUENCES AND OPERON STRUCTURE OF PLASMID-BORNE GENES MEDIATING UPTAKE AND UTILIZATION OF RAFFINOSE IN ESCHERICHIA-COLI [J].

ASLANIDIS, C ;

SCHMID, K ;

SCHMITT, R .

JOURNAL OF BACTERIOLOGY, 1989, 171 (12) :6753-6763

[3] CLONING AND EXPRESSION OF A MEMBER OF THE ASPERGILLUS-NIGER GENE FAMILY ENCODING ALPHA-GALACTOSIDASE [J].

DENHERDER, IF ;

ROSELL, AMM ;

VANZUILEN, CM ;

PUNT, PJ ;

VANDENHONDEL, AMJJ .

MOLECULAR & GENERAL GENETICS, 1992, 233 (03) :404-410

[4] Updating the sequence-based classification of glycosyl hydrolases [J].

Henrissat, B ;

Bairoch, A .

BIOCHEMICAL JOURNAL, 1996, 316 :695-696

[5] Cloning and expression of β-galactosidase gene from Bifidobacterium infantis into Escherichia coli [J].

Hung, MN ;

Lee, BH .

BIOTECHNOLOGY LETTERS, 1998, 20 (07) :659-662

[6]

Johnson John L., 1994, P655

[7]

King MR, 1998, FEMS MICROBIOL LETT, V163, P37, DOI 10.1016/S0378-1097(98)00148-7

[8] α-Galactosidase of Bifidobacterium adolescentis DSM 20083 [J].

Leder, S ;

Hartmeier, W ;

Marx, SP .

CURRENT MICROBIOLOGY, 1999, 38 (02) :101-106

[9] Cloning and nucleotide sequence of the beta-D-glucosidase gene from Bifidobacterium breve clb, and expression of beta-D-glucosidase activity in Escherichia coli [J].

Nunoura, N ;

Ohdan, K ;

Tanaka, K ;

Tamaki, H ;

Yano, T ;

Inui, M ;

Yukawa, H ;

Yamamoto, K ;

Kumagai, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (12) :2011-2018

[10] CLONING AND NUCLEOTIDE-SEQUENCE OF THE ALPHA-GALACTOSIDASE CDNA FROM CYAMOPSIS-TETRAGONOLOBA (GUAR) [J].

OVERBEEKE, N ;

FELLINGER, AJ ;

TOONEN, MY ;

VANWASSENAAR, D ;

VERRIPS, CT .

PLANT MOLECULAR BIOLOGY, 1989, 13 (05) :541-550

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