Tandem Mass Spectrometry with Ultrahigh Mass Accuracy Clarifies Peptide Identification by Database Retrieval

被引：54

作者：

Boyne, Michael T., II ^{[1
]}

Garcia, Benjamin A. ^{[1
]}

Li, Mingxi ^{[1
]}

Zamdborg, Leonid ^{[1
]}

Wenger, Craig D. ^{[1
]}

Babai, Shannee ^{[1
]}

Kelleher, Neil L. ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Post-Translational Modifications; High Resolution; Mass Accuracy; Mass Spectrometry; Proteomics; Human Nuclei; COLLISION-INDUCED DISSOCIATION; TOP-DOWN; PROTEIN IDENTIFICATION; POSTTRANSLATIONAL MODIFICATIONS; KDA PROTEINS; PROTEOMICS; ACQUISITION; MS/MS; MODEL; MS;

D O I：

10.1021/pr800635m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A platform was developed to analyze MS/MS spectra from large peptides with low part-per-million mass accuracy, including a commercial-grade software suite. Termed Middle Down Proteomics, this platform identified 7454 peptides from 2-20 kDa (1472 unique) from 555 proteins after 23 LC-MS/MS injections of Lys-C digests of HeLa-S3 nuclear proteins. Along with greatly increased confidence for both peptide identification (expectation values from 10(-89) to 10(-4)) and characterization (up, to 18% of peptides were modified in some LC-MS/MS runs), fragmentation data with < 2 ppm accuracy enabled error tolerant and routine multiplexed database searching-all clearly demonstrated in this study.

引用

页码：374 / 379

页数：6

共 44 条

[1] The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins [J].