Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Caspofungin Susceptibility Testing of Candida and Aspergillus Species

被引:98
作者
De Carolis, Elena [1 ]
Vella, Antonietta [1 ]
Florio, Ada R. [1 ]
Posteraro, Patrizia [3 ]
Perlin, David S. [4 ]
Sanguinetti, Maurizio [1 ]
Posteraro, Brunella [2 ]
机构
[1] Univ Cattolica Sacro Cuore, Inst Microbiol, Rome, Italy
[2] Univ Cattolica Sacro Cuore, Inst Hyg, Rome, Italy
[3] Osped San Carlo Borromeo Milano, Clin Lab, Rome, Italy
[4] UMDNJ, New Jersey Med Sch, Publ Hlth Res Inst, Newark, NJ USA
关键词
EPIDEMIOLOGIC CUTOFF VALUES; MIC DISTRIBUTIONS; RESISTANCE; ALBICANS; MUTATIONS;
D O I
10.1128/JCM.00224-12
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.
引用
收藏
页码:2479 / 2483
页数:5
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