Sinorhizobium meliloti differentiation during symbiosis with alfalfa:: A transcriptomic dissection

被引:86
作者
Capela, D [1 ]
Filipe, C [1 ]
Bobilk, C [1 ]
Batut, J [1 ]
Bruand, C [1 ]
机构
[1] INRA, CNRS, UMR 441 2594, Lab Interact Plantes Microorganismes, F-31326 Castanet Tolosan, France
关键词
D O I
10.1094/MPMI-19-0363
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sinorhizobium meliloti is a soil bacterium able to induce the formation of nodules on the root of specific legumes, including alfalfa (Medicago sativa). Bacteria colonize nodules through infection threads, invade the plant intracellularly, and ultimately differentiate into bacteroids capable of reducing atmospheric nitrogen to ammonia, which is directly assimilated by the plant. As a first step to describe global changes in gene expression of S. meliloti during the symbiotic process, we used whole genome microarrays to establish the transcriptome profile of bacteria from nodules induced by a bacterial mutant blocked at the infection stage and front wild-type nodules harvested at various timepoints after inoculation. Comparison of these profiles to those of cultured bacteria grown either to log or stationary phase as well as examination of a number of genes with known symbiotic transcription patterns allowed us to correlate global gene-expression patterns to three known steps of symbiotic bacteria bacteroid differentiation, i.e., invading bacteria inside infection threads, young differentiating bacteroids, and fully differentiated, nitrogen-fixing bacteroids. Finally, analysis of individual gene transcription profiles revealed a number of new potential symbiotic genes.
引用
收藏
页码:363 / 372
页数:10
相关论文
共 46 条
[1]   Transcriptome analysis of Sinorhizobium meliloti during symbiosis -: art. no. R15 [J].
Ampe, F ;
Kiss, E ;
Sabourdy, F ;
Batut, J .
GENOME BIOLOGY, 2003, 4 (02)
[2]   Smc01944, a secreted peroxidase induced by oxidative stresses in Sinorhizobium meliloti 1021 [J].
Barloy-Hubler, F ;
Chéron, A ;
Hellégouarch, A ;
Galibert, F .
MICROBIOLOGY-SGM, 2004, 150 :657-664
[3]   A dual-genome Symbiosis Chip for coordinate study of signal exchange and development in a prokaryote-host interaction [J].
Barnett, MJ ;
Tolman, CJ ;
Fisher, RF ;
Long, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (47) :16636-16641
[4]   Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions [J].
Becker, A ;
Bergès, H ;
Krol, E ;
Bruand, C ;
Rüberg, S ;
Capela, D ;
Lauber, E ;
Meilhoc, E ;
Ampe, F ;
de Bruijn, FJ ;
Fourment, J ;
Francez-Charlot, A ;
Kahn, D ;
Küster, H ;
Liebe, C ;
Pühler, A ;
Weidner, S ;
Batut, J .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2004, 17 (03) :292-303
[5]   TE7, AN INEFFICIENT SYMBIOTIC MUTANT OF MEDICAGO-TRUNCATULA GAERTN CV JEMALONG [J].
BENABEN, V ;
DUC, G ;
LEFEBVRE, V ;
HUGUET, T .
PLANT PHYSIOLOGY, 1995, 107 (01) :53-62
[6]   Development of Sinorhizobium meliloti pilot macroarrays for transcriptome analysis [J].
Bergès, H ;
Lauber, E ;
Liebe, C ;
Batut, J ;
Kahn, D ;
de Bruijn, FJ ;
Ampe, F .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (02) :1214-1219
[7]   Analysis of the growth phase-associated transcriptome of Streptococcus pyogenes [J].
Beyer-Sehlmeyer, G ;
Kreikemeyer, B ;
Hörster, A ;
Podbielski, A .
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 295 (03) :161-177
[8]   Chips with everything: DNA microarrays in infectious diseases [J].
Bryant, PA ;
Venter, D ;
Robins-Browne, R ;
Curtis, N .
LANCET INFECTIOUS DISEASES, 2004, 4 (02) :100-111
[9]   Identification of Sinorhizobium meliloti genes regulated during symbiosis [J].
Cabanes, D ;
Boistard, P ;
Batut, J .
JOURNAL OF BACTERIOLOGY, 2000, 182 (13) :3632-3637
[10]   Transcriptome-based identification of the Sinorhizobium meliloti NodD1 regulon [J].
Capela, D ;
Carrere, S ;
Batut, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (08) :4910-4913