Recognition of the codon-anticodon helix by ribosomal RNA

被引：202

作者：

Yoshizawa, S ^{[1
]}

Fourmy, D ^{[1
]}

Puglisi, JD ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

SCIENCE | 1999年 / 285卷 / 5434期

关键词：

D O I：

10.1126/science.285.5434.1722

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Translational fidelity is established by ribosomal recognition of the codon-anticodon interaction within the aminoacyl-transfer RNA (tRNA) site (A site) of the ribosome. Experiments are presented that reveal possible contacts between 16S ribosomal RNA and the codon-anticodon complex. Nf methylation of adenine at position 1492 (A1492) and A1493 interfered with A-site tRNA binding. Mutation of A1492 and A1493 to guanine or cytosine also impaired A-site tRNA binding. The deleterious effects of A1492G or A1493G (or both) mutations were compensated by 2' fluorine substitutions in the mRNA codon. The results suggest that the ribosome recognizes the codon-anticodon complex by adenine contacts to the messenger RNA backbone and provide a mechanism for molecular discrimination of correct versus incorrect codon-anticodon pairs.

引用

页码：1722 / 1725

页数：4

共 39 条

[1] TRANSLATION OF 2'-MODIFIED MESSENGER-RNA IN-VITRO AND IN-VIVO [J].

AURUP, H ;

SIEBERT, A ;

BENSELER, F ;

WILLIAMS, D ;

ECKSTEIN, F .

NUCLEIC ACIDS RESEARCH, 1994, 22 (23) :4963-4968

[2] RRNA chemical groups required for aminoglycoside binding [J].

Blanchard, SC ;

Fourmy, D ;

Eason, RG ;

Puglisi, JD .

BIOCHEMISTRY, 1998, 37 (21) :7716-7724

[3] STRUCTURE OF CHYMOTRYPSIN TRIFLUOROMETHYL KETONE INHIBITOR COMPLEXES - COMPARISON OF SLOWLY AND RAPIDLY EQUILIBRATING INHIBITORS [J].

BRADY, K ;

WEI, AZ ;

RINGE, D ;

ABELES, RH .

BIOCHEMISTRY, 1990, 29 (33) :7600-7607

[4] Small molecule interactions with protein-tyrosine phosphatase PTP1B and their use in inhibitor design [J].

Burke, TR ;

Ye, B ;

Yan, XJ ;

Wang, SM ;

Jia, ZC ;

Chen, L ;

Zhang, ZY ;

Barford, D .

BIOCHEMISTRY, 1996, 35 (50) :15989-15996

[5] STREPTOMYCIN SUPPRESSION + CODE [J].

DAVIES, J ;

GILBERT, W ;

GORINI, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 51 (05) :883-+

[6] Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution [J].

Doublié, S ;

Tabor, S ;

Long, AM ;

Richardson, CC ;

Ellenberger, T .

NATURE, 1998, 391 (6664) :251-258

[7] Paromomycin binding induces a local conformational change in the A-site of 16 S rRNA [J].

Fourmy, D ;

Yoshizawa, S ;

Puglisi, JD .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 277 (02) :333-345

[8] Structure of the A site of Escherichia coli 16S ribosomal RNA complexed with an aminoglycoside antibiotic [J].

Fourmy, D ;

Recht, MI ;

Blanchard, SC ;

Puglisi, JD .

SCIENCE, 1996, 274 (5291) :1367-1371

[9]

GRAJEVSKAJA RA, 1982, EUR J BIOCHEM, V128, P47

[10] Ribosomes and translation [J].

Green, R ;

Noller, HF .

ANNUAL REVIEW OF BIOCHEMISTRY, 1997, 66 :679-716

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