Lipopolysaccharide and tumor necrosis factor-alpha synergy potentiate serum-dependent responses of rat macrophages

被引：3

作者：

Fox, ES ^{[1
]}

Wang, L ^{[1
]}

Tracy, TF ^{[1
]}

机构：

[1] ST LOUIS UNIV,PEDIAT RES INST,SCH MED,DEPT PEDIAT,ST LOUIS,MO 63110

来源：

SHOCK | 1996年 / 5卷 / 06期

关键词：

D O I：

10.1097/00024382-199606000-00007

中图分类号：

R4 [临床医学];

学科分类号：

1002 [临床医学]; 100602 [中西医结合临床];

摘要：

Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) are major mediators of sepsis and multiple organ failure. Serum-mediated macrophage activation requires lipopolysaccharide (LPS) and its serum binding protein, lipopolysaccharide binding protein as a ligand for the receptor CD14, This study was designed to determine whether cytokines participate in regulation of serum-mediated LPS activation. Rat macrophages were stimulated with LPS with and without TNF-alpha or IL-1 beta and activation was determined by detection of TNF-alpha by specific enzyme-linked immunosorbent assay or TNF-alpha mRNA by Northern blot analysis. The addition of TNF-alpha but not IL-1 beta, in the presence of serum, leads to potentiation of macrophage activation after LPS stimulation. This effect could be specifically inhibited by neutralization of LPS with polymyxin B or an antibody against TNF-alpha. This study shows that LPS and TNF-alpha synergize to potentiate serum-mediated macrophage activation, These results demonstrate another element of the control mechanism of cytokine secretion following macrophage activation in sepsis.

引用

页码：429 / 433

页数：5

共 22 条

[1]

A CDNA SEQUENCE ENCODING CYTOSKELETAL GAMMA-ACTIN FROM RAT [J].

BROWN, CW ;

MCHUGH, KM ;

LESSARD, JL .

NUCLEIC ACIDS RESEARCH, 1990, 18 (17) :5312-5312

[2]

IDENTIFICATION OF A COMMON NUCLEOTIDE-SEQUENCE IN THE 3'-UNTRANSLATED REGION OF MESSENGER-RNA MOLECULES SPECIFYING INFLAMMATORY MEDIATORS [J].

CAPUT, D ;

BEUTLER, B ;

HARTOG, K ;

THAYER, R ;

BROWNSHIMER, S ;

CERAMI, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (06) :1670-1674

[3]

DENTENER MA, 1993, J IMMUNOL, V151, P4258

[4]

Alterations in tumor necrosis factor-alpha expression by hepatic macrophages following acute cholestatic liver injury [J].

Fox, ES ;

Tracy, TF .

SHOCK, 1996, 5 (02) :112-115

[5]

LIPOPOLYSACCHARIDE-BINDING PROTEIN AS A MAJOR PLASMA-PROTEIN RESPONSIBLE FOR ENDOTOXEMIC SHOCK [J].

GALLAY, P ;

HEUMANN, D ;

LEROY, D ;

BARRAS, C ;

GLAUSER, MP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :9935-9938

[6]

MODE OF ACTION OF ANTI-LIPOPOLYSACCHARIDE-BINDING PROTEIN ANTIBODIES FOR PREVENTION OF ENDOTOXEMIC SHOCK IN MICE [J].

GALLAY, P ;

HEUMANN, D ;

LEROY, D ;

BARRAS, C ;

GLAUSER, MP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (17) :7922-7926

[7]

GELLER DA, 1993, ARCH SURG-CHICAGO, V128, P22

[8]

TRANSFORMING GROWTH FACTOR-BETA(1) LOWERS THE CD14 CONTENT OF MONOCYTES [J].

HAMON, G ;

MULLOY, RH ;

CHEN, G ;

CHOW, R ;

BIRKENMAIER, C ;

HORN, JK .

JOURNAL OF SURGICAL RESEARCH, 1994, 57 (05) :574-578

[9]

INTERLEUKIN-4 DOWN-REGULATES THE EXPRESSION OF CD14 IN NORMAL HUMAN MONOCYTES [J].

LAUENER, RP ;

GOYERT, SM ;

GEHA, RS ;

VERCELLI, D .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1990, 20 (11) :2375-2381

[10]

THE RECOMBINANT 23-KDA N-TERMINAL FRAGMENT OF BACTERICIDAL PERMEABILITY-INCREASING PROTEIN (RBPI(23)) DECREASES ESCHERICHIA COLI-INDUCED MORTALITY AND ORGAN INJURY DURING IMMUNOSUPPRESSION-RELATED NEUTROPENIA [J].

LECHNER, AJ ;

LAMPRECH, KE ;

JOHANNS, CA ;

MATUSCHAK, GM .

SHOCK, 1995, 4 (04) :298-306

← 1 2 3 →