Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis

被引：26

作者：

Scarpellini, P

Braglia, S

Carrera, P

Cedri, M

Cichero, P

Colombo, A

Crucianelli, R

Gori, A

Ferrari, M

Lazzarin, A

机构：

[1] San Raffaele Sci Inst, Div Infect Dis, I-20127 Milan, Italy

[2] San Raffaele Sci Inst, Clin Mol Biol Lab, I-20127 Milan, Italy

[3] San Raffaele Sci Inst, Microbiol Lab, I-20127 Milan, Italy

[4] Univ Milan, Clin Infect Dis, Milan, Italy

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 10期

关键词：

D O I：

10.1128/AAC.43.10.2550

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

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页码：2550 / 2554

页数：5

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