Alternative usage of exon 1 of bovine PrP mRNA

被引：17

作者：

Horiuchi, M ^{[1
]}

Ishiguro, N ^{[1
]}

Nagasawa, H ^{[1
]}

Toyoda, Y ^{[1
]}

Shinagawa, M ^{[1
]}

机构：

[1] OBIHIRO UNIV AGR & VET MED,INST PROTOZOAN MOL IMMUNOL,OBIHIRO,HOKKAIDO 080,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 233卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1006/bbrc.1997.6511

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report two types of bovine prion protein (PrP) mRNA that possessed different lengths of the 5'-untranslated region and were expressed in various bovine tissues,The two mRNA species were transcribed from identical positions but differed in the usage of the splice site for exon 1/intron, One mRNA possessed exon 1 consisting of 53 nucleotides and the other possessed exon 1 consisting of 168 nucleotides. Usage of exons 2 and 3 was identical for the two mRNA species, The two mRNA species were detected in all but spleen tissue; the mRNA possessing 168-nt exon 1 was mot detected in bovine spleen, This is the first report on the tissue-specific alternative splicing of PrP mRNA in any other species, Only a low level of PrPc appeared to be present in bovine spleen, These results suggested the possibility that the mRNA possessing 53-nt exon 1 was inefficiently translated into Prp(c); however, in vitro translation analysis showed no marked difference in translational efficiency between the two mRNA species. (C) 1997 Academic Press.

引用

页码：650 / 654

页数：5

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