Cloning and characterization of mycovirus double-stranded RNA from the plant pathogenic fungus, Fusarium solani f sp robiniae

被引：45

作者：

Nogawa, M ^{[1
]}

Kageyama, T ^{[1
]}

Nakatani, A ^{[1
]}

Taguchi, G ^{[1
]}

Shimosaka, M ^{[1
]}

Okazaki, M ^{[1
]}

机构：

[1] SHINSHU UNIV, FAC TEXT SCI & TECHNOL, DEPT APPL BIOL, UEDA, NAGANO 386, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 05期

关键词：

cDNA synthesis; nucleotide sequence; in vitro replication; RNA-dependent RNA polymerase; capsid protein;

D O I：

10.1271/bbb.60.784

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mycovirus (named FusoV) from the phytopathogenic fungus, Fusarium solani f. sp. robiniae SUF704, has two kinds of double-stranded (ds) RNA genomes, designated M1 and M2. The cDNAs were constructed from FusoV genomic dsRNAs, The sequences of M1 and M2 cDNAs comprised 1645 and 1445bp, respectively, Sequence analysis showed that each dsRNA had a single long open reading frame (ORF) on only one of the strands, M1 ORF encodes a 519-amino acid residue polypeptide with a predicted molecular mass of 60 kDa, RNA-dependent RNA polymerase-conserved motifs were identified in the predicted amino acid sequence, and the polymerase synthesized dsRNA in vitro, The M2 ORF encodes a polypeptide of 413 amino acid residues with a predicted molecular mass of 44 kDa, The predicted amino acid sequence contained the sequence corresponding to those found in the purified 44-kDa capsid protein of FusoV.

引用

页码：784 / 788

页数：5

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