Effect of the spacer arm in affinity chromatography:: Determination of adsorption characteristics and flow rate effect

被引:12
作者
del Valle, EMM [1 ]
Galán, MA [1 ]
机构
[1] Univ Salamanca, Dept Ingn Quim, E-37008 Salamanca, Spain
关键词
D O I
10.1021/ie0106884
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The effect of the spacer arm in affinity chromatography based on the adsorption equilibrium of L-asparaginase on activated Sepharose 4B with aliphatic diamines (1,4-diaminobutane-1,8-diaminooctane) as the spacer arms and L-(+)-chlorosuccinamic acid as the ligand was studied in a batch reactor and in a packed bed. The adsorption equilibrium constants were determined for all adsorbents. A maximum in the adsorption was observed when 1,6-diaminohexane was used as the spacer arm under optimum adsorption conditions (pH 8.6, 298 K, and 0.05 M NaCl) (Martin del Valle, E. M.; Galan, M. A. Ind. Eng. Chem. Res. 2001, 40 (1), 369-376). Partition coefficients were also obtained in the batch reactor. The values of these coefficients allowed us to quantify the amounts of specifically adsorbed, nonspecifically adsorbed, and occluded enzyme. A pulse-response chromatographic method and statistical moments were used to determine the adsorption equilibrium constants in the packed bed. The values of the adsorption equilibrium constants for different adsorbents were compared with those obtained previously in the batch reactor taking into account the mass-transfer resistance inside the particles of the adsorbent (partition coefficient). The difference in the values obtained was lower than 5-8%. In addition, the effect of the flow rate on adsorption was studied for different adsorbents with spacer arms of different lengths. When the flow rate was increased, the value of the adsorption equilibrium constant increased. This effect was observed when 1,8-diaminooctane was used as the spacer arm.
引用
收藏
页码:2296 / 2304
页数:9
相关论文
共 21 条
[1]  
ARRANZ MA, 1981, ING QUIM, V11, P125
[2]   Adsorption equilibrium of α-amylase in aqueous solutions [J].
Bautista, LF ;
Martínez, M ;
Aracil, J .
AICHE JOURNAL, 1999, 45 (04) :761-768
[3]  
CANTERO D, 1987, AN QUIM, V83, P641
[4]  
Deen W. M., 1998, ANAL TRANSPORT PHENO
[5]   Specific and nonspecific adsorption in affinity chromatography.: Part II.: Kinetic and mass transfer studies [J].
del Valle, EMM ;
Galán, MA .
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2001, 40 (01) :377-383
[6]   Specific and nonspecific adsorption in affinity chromatography.: Part I.: Preliminary and equilibrium studies [J].
del Valle, EMM ;
Galán, MA .
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2001, 40 (01) :369-376
[7]  
DITKIN VA, 1951, SPRAVOCHNIK OPERACIO
[8]   AFFINITY-CHROMATOGRAPHY - EFFECT OF PARTICLE-SIZE ON ADSORPTION EQUILIBRIUM AND MASS-TRANSFER KINETICS [J].
GONZALEZPATINO, F ;
CATALAN, J ;
GALAN, MA .
CHEMICAL ENGINEERING SCIENCE, 1993, 48 (09) :1567-1573
[9]  
HERMANSON TG, 1992, IMMOBILIZED AFFINITY, P4
[10]  
HOLMBERG B, 1926, CHEM BER, V59, P1569