Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced

被引：5

作者：

van Hijum, SAFT

Bonting, K

van der Maarel, MJEC

Dijkhuizen, L

机构：

[1] Univ Groningen, Gronigen Biomol Sci & Biotechnol Inst, Microbial Physiol Res Grp, NL-9750 AA Haren, Netherlands

[2] TNO Nutr & Food Res, Carbohydrate Technol Dept, NL-9723 CC Groningen, Netherlands

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 205卷 / 02期

关键词：

levan; levansucrase; Lactobacillus reuteri;

D O I：

10.1016/S0378-1097(01)00490-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

引用

页码：323 / 328

页数：6

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