Resveratrol inhibits nonalcoholic fatty liver disease in rats

被引:191
作者
Bujanda, Luis [1 ]
Hijona, Elizabeth [1 ]
Larzabal, Mikel [2 ]
Beraza, Marta [1 ]
Aldazabal, Pablo [3 ]
Garcia-Urkia, Nerea [3 ]
Sarasqueta, Cristina [4 ]
Cosme, Angel [1 ]
Irastorza, Belen [5 ]
Gonzalez, Alberto [6 ]
Arenas, Juan I., Jr. [1 ]
机构
[1] Univ Basque Country, Dept Gastroenterol, Donostia Hosp, CIBERehd, San Sebastian, Spain
[2] Donostia Hosp, Dept Pathol, San Sebastian, Spain
[3] Donostia Hosp, Dept Expt Surg, San Sebastian, Spain
[4] Donostia Hosp, Dept Epidemiol, San Sebastian, Spain
[5] Donostia Hosp, Dept Pharmacol, San Sebastian, Spain
[6] Donostia Hosp, Dept Microbiol, San Sebastian, Spain
关键词
D O I
10.1186/1471-230X-8-40
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: The prevalence of nonalcoholic fatty liver disease (NAFLD) is high. NAFLD is linked to obesity, diabetes mellitus, and hypertriglyceridemia. Approximately 20% of patients with NAFLD will eventually develop cirrhosis. Our purpose was to investigate whether resveratrol decreased hepatic steatosis in an animal model of steatosis, and whether this therapeutic approach resulted in a decrease in tumor necrosis factor alpha (TNF-alpha) production, lipid peroxidation and oxidative stress. Methods: Male Wistar CRL: Wi (Han) (225 g) rats were randomized into three groups. A control group (n = 12) was given free access to regular dry rat chow for 4 weeks. The steatosis (n = 12) and resveratrol (n = 12) groups were given free access to feed (a high carbohydrate-fat free modified diet) and water 4 days per week, and fasted for the remaining 3 days for 4 weeks. Rats in the resveratrol group were given resveratrol 10 mg daily by the oral route. All rats were killed at 4 weeks and assessed for fatty infiltration and bacterial translocation. Levels of TNF-alpha in serum, hepatic malondialdehyde (MDA), oxidative stress (superoxide dismutase, glutathione peroxidase, catalase and nitric oxide synthase) and biochemical parameters were measured. Results: Fat deposition was decreased in the resveratrol group as compared to the steatosis group (Grade 1 vs Grade 3, P < 0.05). TNF-alpha and MDA levels were significantly increased in the steatosis group (TNF-alpha; 33.4 +/- 5.2 vs 26.24 +/- 3.47 pg/ml and MDA; 9.08 +/- 0.8 vs 3.17 +/- 1.45 mu M respectively, P < 0.05). This was accompanied by increased superoxide dismutase, glutathione peroxidase and catalase and decreased nitric oxide synthase in the liver of resveratrol group significantly (P < 0.05 vs steatosis group). Bacterial translocation was not found in any of the groups. Glucose levels were decreased in the group of rats given resveratrol (P < 0.05). Conclusion: Resveratrol decreased NAFLD severity in rats. This effect was mediated, at least in part, by TNF-alpha inhibition and antioxidant activities.
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页数:8
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