Neuroprotective Effect of Atorvastatin Involves Suppression of TNF-α and Upregulation of IL-10 in a Rat Model of Intracerebral Hemorrhage

被引：94

作者：

Tu Ewen ^{[1
]}

Liu Qiuting ^{[1
]}

Tang Chaogang ^{[1
]}

Tang Tao ^{[1
]}

Wu Jun ^{[1
]}

Tan Liming ^{[1
]}

Xiang Guanghong ^{[1
]}

机构：

[1] Brains Hosp Hunan Prov, Dept Neurol, Changsha 410007, Hunan, Peoples R China

来源：

CELL BIOCHEMISTRY AND BIOPHYSICS | 2013年 / 66卷 / 02期

关键词：

Experimental-intracerebral hemorrhage; Atorvastatin; Acute inflammatory reaction; TNF-alpha; IL-10; STATINS;

D O I：

10.1007/s12013-012-9453-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

We evaluated the neuroprotective effects of atorvastatin (2, 5, and 10 mg/kg) on experimentally induced intracerebral hemorrhage (ICH) in adult rats; controls were administered PBS. Plasma TNF-alpha and IL-10 levels before and after ICH were analyzed at various time points by enzyme-linked immunosorbent assay (ELISA) and neurological behavior of rats was assessed by climbing scores. At 3-days postoperatively, brain water contents and TNF-alpha/IL-10 expression in brain tissue were determined. Histopathological changes and microglial cells in the brain tissue were evaluated by light-microscopy. Post-ICH neurological deficits differed significantly between sham-operated group A and experimental-ICH group B (P < 0.05). Brain water contents were significantly less in group A than in group B (P < 0.05). Significant differences (P < 0.05) between two groups were observed regarding activated microglia, TNF-alpha and IL-10 levels. Compared with group B, neurological deficits, brain water contents, pathological changes, and activated microglia were reduced (P < 0.05) in groups C (Experimental-ICH + atorvastatin 2 mg/kg), D (Experimental-ICH + atorvastatin 5 mg/kg) and E (Experimental-ICH + atorvastatin 10 mg/kg). Atorvastatin-induced a dose-dependent reduction of TNF-alpha and increase of IL-10 levels (P < 0.05). Therefore, it was concluded that atorvastatin improved neurofunctional rehabilitation in rats through the suppression of cytokines-mediated inflammatory response and attenuation of brain damage following intracerebral hemorrhage.

引用

页码：337 / 346

页数：10

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