A MALDI TOF MS-based minisequencing method for rapid detection of TEM-type extended-spectrum beta-lactamases in clinical strains of Enterobacteriaceae

被引：32

作者：

Ikryannikova, L. N. ^{[1
]}

Shitikov, E. A. ^{[1
]}

Zhivankova, D. G. ^{[1
]}

Il'ina, E. N. ^{[1
]}

Edelstein, M. V. ^{[2
]}

Govorun, V. M. ^{[1
]}

机构：

[1] Russian Federat, Minist Publ Hlth, Res Inst Phys Chem Med, Moscow 119992, Russia

[2] Inst Antimicrobial Chemotherapy, Smolensk, Russia

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2008年 / 75卷 / 03期

关键词：

TEM beta-lactamase; Enterobacteriaceae; MALDI TOF mass spectrometry; Minisequencing;

D O I：

10.1016/j.mimet.2008.07.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A minisequencing method based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) was developed for rapid identification of single nucleotide polymorphisms at bla(TEM) gene codons 104, 164 and 238 associated with extended-spectrum activity on TEM-type betalactamases. The method was validated by testing the Escherichia coli and Klebsiella pneumoniae strains possessing the known blaTEM gene sequences. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：385 / 391

页数：7

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