Multiple isomorphous replacement on merohedral twins:: structure determination of deacetoxycephalosporin C synthase

被引：15

作者：

van Scheltinga, ACT

Valegård, K

Ramaswamy, S

Hajdu, J

Andersson, I

机构：

[1] Swedish Univ Agr Sci, Dept Mol Biol, S-75124 Uppsala, Sweden

[2] Uppsala Univ, Dept Biochem, S-75123 Uppsala, Sweden

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2001年 / 57卷

关键词：

D O I：

10.1107/S0907444901014081

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Merohedral twinning is a packing anomaly that seriously impairs the determination of macromolecular crystal structures. Crystals of deacetoxycephalosporin C synthase (DAOCS), an enzyme involved in the expansion of the penicillin nucleus to form the core structure of the cephalosporin antibiotics, were found to be merohedrally twinned by many diagnostic criteria. Here, the structure determination of DAOCS from twinned crystals based on a combination of isomorphous replacement and the use of a multiple-wavelength diffraction data set is described.

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页码：1776 / 1785

页数：10

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