Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA

被引：88

作者：

Verkaar, ELC ^{[1
]}

Nijman, IJ ^{[1
]}

Boutaga, K ^{[1
]}

Lenstra, JA ^{[1
]}

机构：

[1] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, NL-3508 TD Utrecht, Netherlands

来源：

MEAT SCIENCE | 2002年 / 60卷 / 04期

关键词：

species identification; PCR-RFLP; mitochondrial DNA; satellite DNA; bovini; beef;

D O I：

10.1016/S0309-1740(01)00144-9

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Methods currently used for the identification of the species origin of meat or tissue samples have not been validated for other bovine species than taurine cattle or water buffalo. These methods also do not discriminate between the different bovine species that are used as source of beef. Here. we describe two complementary methods for detection and differentiation of bovine species, which are based on mutations in mitochondrial DNA and centromeric satellite DNA, respectively. The analysis of satellite DNA is especially relevant for the identification of animals that are of hybrid origin. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：365 / 369

页数：5

共 31 条

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