Epicatechin protects auditory cells against cisplatin-induced death

被引：43

作者：

Kim, Chul-Ho ^{[1
,2
]}

Kang, Sung Un ^{[1
,2
]}

Pyun, JungHee ^{[1
,2
]}

Lee, Mi Hye ^{[1
,2
]}

Hwang, Hye Sook ^{[1
,2
]}

Lee, HaNeul ^{[1
]}

机构：

[1] Ajou Univ, Sch Med, Dept Otolaryngol, Suwon 442749, South Korea

[2] Ajou Univ, Sch Med, Ctr Cell Death Regulating Biodrugs, Suwon 442749, South Korea

来源：

APOPTOSIS | 2008年 / 13卷 / 09期

关键词：

Ototoxicity; Cisplatin; Epicatechin; Hearing loss; Apoptosis;

D O I：

10.1007/s10495-008-0242-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cisplatin, a chemotherapeutic drug that is widely used to treat various cancers, promotes ototoxicity at higher doses. In this study, the effect of epicatechin (EC) on cisplatin-induced hair cell death was investigated in a cochlear organ of Corti-derived cell line, HEI-OC1, and in vivo in zebrafish. Cisplatin promoted apoptosis and altered mitochondrial membrane potential (MMP) in HEI-OC1 cells. EC inhibited cisplatin-induced apoptosis and intracellular reactive oxygen species (ROS) generation. Labeling of zebrafish lateral line hair cells by the fluorescent dye YO-PRO1 was lost upon exposure to cisplatin, and EC protected against this cisplatin-induced loss of labeling in a dose-dependent manner. Scanning and transmission electron micrographs showed that treatment with EC protected against cisplatin-induced loss of kinocilium and stereocilia in zebrafish neuromasts. These results suggest that EC prevents cisplatin-induced ototoxicity by blocking ROS generation and by preventing changes in MMP.

引用

页码：1184 / 1194

页数：11

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