Deficiency in Acellular Cementum and Periodontal Attachment in Bsp Null Mice

被引：99

作者：

Foster, B. L. ^{[1
]}

Soenjaya, Y. ^{[2
]}

Nociti, F. H., Jr. ^{[1
,3
]}

Holm, E. ^{[4
]}

Zerfas, P. M. ^{[5
]}

Wimer, H. F. ^{[6
]}

Holdsworth, D. W. ^{[7
]}

Aubin, J. E. ^{[8
,9
]}

Hunter, G. K. ^{[2
,10
]}

Goldberga, H. A. ^{[2
,4
,10
]}

Somermana, M. J. ^{[1
]}

机构：

[1] NIAMSD, NIH, Bethesda, MD 20892 USA

[2] Univ Western Ontario, Schulich Sch Med & Dent, Biomed Engn Program, London, ON, Canada

[3] Univ Estadual Campinas, Sch Dent, Div Periodont, Dept Prosthodont & Periodont, Piracicaba, SP, Brazil

[4] Univ Western Ontario, Sch Med & Dent, Dept Biochem, London, ON, Canada

[5] NIH, Div Vet Resources, Off Res Serv, Bethesda, MD 20892 USA

[6] Smithsonian Inst, Dept Vertebrate Zool, Natl Museum Nat Hist, Washington, DC 20560 USA

[7] Robarts Res Inst, Imaging Res Labs, London, ON N6A 5C1, Canada

[8] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada

[9] Univ Toronto, Fac Dent, Toronto, ON, Canada

[10] Univ Western Ontario, Sch Dent, Schulich Sch Med & Dent, London, ON, Canada

来源：

JOURNAL OF DENTAL RESEARCH | 2013年 / 92卷 / 02期

基金：

美国国家卫生研究院; 加拿大健康研究院;

关键词：

bone sialoprotein; cementum; periodontal ligament; bone; alkaline phosphatase; extracellular matrix; BONE SIALOPROTEIN; HYDROXYAPATITE; OSTEOPONTIN; IDENTIFICATION; INHIBITION; NUCLEATION; EXPRESSION; PROTEINS; DENTIN;

D O I：

10.1177/0022034512469026

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

Bone sialoprotein (BSP) is an extracellular matrix protein found in mineralized tissues of the skeleton and dentition. BSP is multifunctional, affecting cell attachment and signaling through an RGD integrin-binding region, and acting as a positive regulator for mineral precipitation by nucleating hydroxyapatite crystals. BSP is present in cementum, the hard tissue covering the tooth root that anchors periodontal ligament (PDL) attachment. To test our hypothesis that BSP plays an important role in cementogenesis, we analyzed tooth development in a Bsp null ((-/-)) mouse model. Developmental analysis by histology, histochemistry, and SEM revealed a significant reduction in acellular cementum formation on Bsp(-/-) mouse molar and incisor roots, and the cementum deposited appeared hypomineralized. Structural defects in cementum-PDL interfaces in Bsp(-/-) mice caused PDL detachment, likely contributing to the high incidence of incisor malocclusion. Loss of BSP caused progressively disorganized PDL and significantly increased epithelial down-growth with aging. Bsp(-/-) mice displayed extensive root and alveolar bone resorption, mediated by increased RANKL and the presence of osteoclasts. Results collected here suggest that BSP plays a non-redundant role in acellular cementum formation, likely involved in initiating mineralization on the root surface. Through its importance to cementum integrity, BSP is essential for periodontal function.

引用

页码：166 / 172

页数：7

共 32 条

[1]

Phosphorylation of Ser136 is critical for potent bone sialoprotein-mediated nucleation of hydroxyapatite crystals [J].