Passive immunization with antiserum to a nontoxic alpha-toxin mutant from Staphylococcus aureus is protective in a murine model

被引：80

作者：

Menzies, BE ^{[1
]}

Kernodle, DS ^{[1
]}

机构：

[1] DEPT VET AFFAIRS MED CTR, NASHVILLE, TN 37212 USA

来源：

INFECTION AND IMMUNITY | 1996年 / 64卷 / 05期

关键词：

D O I：

10.1128/IAI.64.5.1839-1841.1996

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A nonhemolytic, nonlethal variant of Staphylococcus aureus alpha-toxin constructed via oligonucleotide-directed mutagenesis and containing a single amino acid substitution (H-35 to L) was used to immunize a rabbit. The resulting antiserum was cross-reactive with wild-type alpha-toxin and neutralized its hemolytic activity in vitro. Passive immunization of mice,vith rabbit antiserum conferred protection against lethal challenge with wild-type alpha-toxin and against acute lethal challenge with a high-alpha-toxin-producing S. aureus strain. H35L alpha-toxin may be useful as a protective immunogen in S. aureus vaccine studies.

引用

页码：1839 / 1841

页数：3

共 20 条

[1] EFFECT OF IMMUNIZATION WITH HIGHLY PURIFIED ALPHA-TOXINS AND BETA-TOXINS ON STAPHYLOCOCCAL MASTITIS IN RABBITS [J].