Recovery of extracellular inulinase by expanded bed adsorption

被引：16

作者：

Pessoa, A

Hartmann, R

Vitolo, M

Hustedt, H

机构：

[1] FAENQUIL,CTR BIOTECNOL,BR-12600000 LORENA,SP,BRAZIL

[2] UNIV SAO PAULO,FCF CP,BR-66083 SAO PAULO,BRAZIL

[3] GESELL BIOTECHNOL FORSCH MBH,D-38124 BRAUNSCHWEIG,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1996年 / 51卷 / 01期

关键词：

expanded-bed adsorption; inulinase; Candida kefyr;

D O I：

10.1016/0168-1656(96)01573-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Adsorption of inulinase onto STREAMLINE (TM) ion-exchange adsorbents in the absence and presence of Candida kefyr cells has been studied. The favourable adsorption pH was in the range of 6.5 and 7.0 using the weak anion exchanger (DEAE). In the case of the strong cation exchanger (SP), pH 4.0 was found to be appropriate. The adsorption isotherms fitted to the Langmuir equation well. Expanded bed adsorption performed with original cultivation medium showed enzyme recovery of 93%. The enrichment and the concentration factors were 5.8 and 2.8, respectively. The presence of cells did not cause problems.

引用

页码：89 / 95

页数：7

共 11 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] CONTINUOUS AFFINITY-CHROMATOGRAPHY USING A MAGNETICALLY STABILIZED FLUIDIZED-BED [J].