Determination of thymol in human plasma by automated headspace solid-phase microextraction-gas chromatographic analysis

被引:57
作者
Kohlert, C
Abel, G
Schmid, E
Veit, M
机构
[1] Zent Inst Arzneimittelforsch GmbH, D-53489 Sinzig, Germany
[2] Bionorica AG, D-92318 Neumarkt Opf, Germany
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 767卷 / 01期
关键词
thyme; Bioavailability; thymol;
D O I
10.1016/S0378-4347(01)00518-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A reliable and sensitive method was developed for determination of thymol in human plasma by automated headspace solid-phase microextraction (SPME). After enzymatic cleavage of thymol sulfate thymol was extracted by a 65 mum polydimethylsiloxane-divinylbenzene crimped fiber (Supelco) after addition of sodium chloride and phosphoric acid (85%). Desorption of the fiber was performed in the injection port of a gas chromatograph at 220degreesC (HP 5890; 50 mX0.2 mm. I.D., 0.2 mum HP Innowax capillary column; flame ionization detection). Fibers were used repeatedly up to 40 analysis. The recovery was 5% after 35 min of extraction. The calibration curve was linear in the range of 8.1-203.5 ng ml(-1) with a limit of quantitation (LOQ) of 8.1 ng ml(-1). The within-day and between-day precision and. accuracy were less than or equal to20% at the LOQ and <15% at higher concentrations according to international guidelines for validation of bioanalytical methods. After administration of a thymol-containing herbal extract only thymol sulfate, no free thymol, could be detected in human plasma, thus analysis of thymol was after enzymatic cleavage of thymol sulfate. It is concluded that the newly developed automated method can be used in clinical trials on bioavailability and pharmacokinetics of thymol-containing herbal medicinal products. (C) 2002 Elsevier Science BV All rights reserved.
引用
收藏
页码:11 / 18
页数:8
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