Computer screen photo-assisted detection of complementary DNA strands using a luminescent zwitterionic polythiophene derivative

被引：21

作者：

Filippini, D ^{[1
]}

Åsberg, P ^{[1
]}

Nilsson, P ^{[1
]}

Inganäs, O ^{[1
]}

Lundström, I ^{[1
]}

机构：

[1] Linkoping Univ, IFM, SE-58183 Linkoping, Sweden

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2006年 / 113卷 / 01期

关键词：

computer screen photo-assisted techniques; complementary DNA detection; POWT; biosensing; fluorescence fingerprinting;

D O I：

10.1016/j.snb.2005.03.112

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The computer screen photo-assisted technique (CSPT), a practical method for the evaluation of assays using computer screens as light sources and web cameras as detectors, has been used to detect the attachment of complementary DNA strands (20-mer. 5'-CAT GAT TGA ACC ATC CAC CA-3') to a complex of single DNA strands and a polythiophene derivative (poly(3-[(S)-5-amino-5-carboxyl-3-oxapentyl]-2.5-thiophenylene hydrochloride (POWT)). The complex is a highly sequence specific indicator. based On non-covalent coupling of DNA to a water-soluble, zwitterionic, electroactive and photoactive polymer able to produce a combined absorption-emmission signal readable by CSPT. The observed CSPT signal retains key spectral features of the complex spectrum, distinguishing the DNA attachment, as well as other stimuli, such as pH regulation at concentrations of 30 mu M POWT and 15 mu M DNA. A CSPT time resolved (linked to temperatures between 8 and 18 C) approach is demonstrated as a complementary source of discrimination and for testing the robustness of the achieved classification. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：410 / 418

页数：9

共 17 条

[1]

ANDERSSON M, 1991, POLYM COMMUN, V32, P546

[2] Controlling inter-chain and intra-chain excitations of a poly(thiophene) derivative in thin films [J].