Chlorpromazine and sodium dodecyl sulfate mixed micelles investigated by small angle X-ray scattering

被引:53
作者
Caetano, W
Gelamo, EL
Tabak, M
Itri, R
机构
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Sao Paulo, Inst Fis, BR-05315970 Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
chlorpromazine (CPZ); sodium dodecyl sulfate (SDS) micelles; small-angle X-ray scattering (SAXS); drug-micelle interaction;
D O I
10.1006/jcis.2001.8164
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Small-angle X-ray scattering (SAXS) studies are reported on the interaction of chlorpromazine (CPZ) with micelles of anionic surfactant sodium dodecyl sulfate (SDS). Isotropic solutions of SDS (40 and 100 mM) at pH 4.0, 7.0, and 9.0 in the absence and presence of CPZ (2-25 mM) were investigated at the National Laboratory of Synchrotron Light (LNLS, Campinas, Brazil). The data were analyzed through the modeling of the micellar form factor and interference function. The results evidence a micellar shape transformation from prolate ellipsoid to cylinder accompanied by micellar growth and surface charge screening as the molar ratio CPZ : SDS increases in the complex. Small ellipsoids with axial ratio v =1.5 +/- 0.1 at 40 mM SDS grow and reassemble into cylinder-like aggregates upon 5 mM drug incorporation (1 CPZ : 8 SDS monomers) with a decrease of the micelle surface charge. At 10 mM CPZ : 40 mM SDS cylindrical micelles are totally screened with an axial ratio v approximate to 2.5. The data also indicate the presence of small prolate ellipsoids (v =1.7 +/- 0.1) in solutions of 100 mM SDS (no drug) and micellar growth (v approximate to 2.0 and 4.0) when 10 and 25 mM CPZ are added to the system. In the latter case, the aggregate is also better represented by a cylinder-like form. Therefore, our results demonstrate that the axial ratio and shape evolution of the surfactant: phenothiazine complex are both SDS concentration and drug: SDS molar ratio dependent. The drug location close to the SDS polar headgroup region without disrupting in a significant way both the paraffinic hydrophobic core and the polar shell thickness is inferred. SAXS data made it possible to obtain the shapes and dimensions of CPZ/SDS aggregates (C) 2002 Elsevier Science (USA).
引用
收藏
页码:149 / 157
页数:9
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