Increased Detectability of Plasma HIV-1 RNA after Introduction of a New Assay and Altered Specimen-Processing Procedures

被引：22

作者：

Rebeiro, Peter F. ^{[1
]}

Kheshti, Asghar ^{[1
,4
]}

Bebawy, Sally S. ^{[1
]}

Stinnette, Samuel E. ^{[1
]}

Erdem, Husamettin ^{[1
]}

Tang, Yi-Wei ^{[1
,2
]}

Sterling, Timothy R. ^{[1
,3
]}

Raffanti, Stephen P. ^{[1
,4
]}

D'Aquila, Richard T. ^{[1
]}

机构：

[1] Vanderbilt Univ, Med Ctr, Sch Med, Div Infect Dis,Dept Med, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Sch Med, Mol Infect Dis Lab, Dept Pathol, Nashville, TN 37232 USA

[3] Vanderbilt Univ, Sch Med, Ctr Hlth Serv Res, Nashville, TN 37232 USA

[4] Comprehens Care Ctr, Nashville, TN USA

来源：

CLINICAL INFECTIOUS DISEASES | 2008年 / 47卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1086/592693

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

After changes to assay and specimen-processing methods, plasma human immunodeficiency virus type 1 (HIV-1) RNA was frequently detectable in patients who previously had well-suppressed HIV-1 RNA levels. This artifact is attributable to shipping frozen plasma in primary plasma preparation tubes and is not caused by the HIV-1 RNA detection assay; it can be avoided by shipping plasma in a secondary tube.

引用

页码：1354 / 1357

页数：4

共 12 条

[1]

*AIDS CLIN TRIALS, 2008, CLIN TRIALS TRIALS O

[2]

*CDCP, 2001, MMWR RECOMM REP, V50, P63

[3] Elevation of viral load by PCR and use of plasma preparation tubes for quantification of human immunodeficiency virus type 1 [J].

Garcia-Bujalance, S. ;

Ladron de Guevara, C. ;

Gonzalez-Garcia, J. ;

Arribas, J. R. ;

Zamora, F. ;

Gutierrez, A. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2007, 69 (02) :384-386

[4] Increased levels of HIV RNA detected in samples with viral loads close to the detection limit collected in Plasma Preparation Tubes™ (PPT) [J].

Griffith, BP ;

Mayo, DR .

JOURNAL OF CLINICAL VIROLOGY, 2006, 35 (02) :197-200

[5] Effect of adherence to newly initiated antiretroviral therapy on plasma viral load [J].

Gross, R ;

Bilker, WB ;

Friedman, HM ;

Strom, BL .

AIDS, 2001, 15 (16) :2109-2117

[6] Plasma viral load and CD4(+) lymphocytes as prognostic markers of HIV-1 infection [J].

Mellors, JW ;

Munoz, A ;

Giorgi, JV ;

Margolick, JB ;

Tassoni, CJ ;

Gupta, P ;

Kingsley, LA ;

Todd, JA ;

Saah, AJ ;

Detels, R ;

Phair, JP ;

Rinaldo, CR .

ANNALS OF INTERNAL MEDICINE, 1997, 126 (12) :946-954

[7]

Roland ME, 2006, AIDS READ, V16, P675

[8]

Roland Michelle E, 2006, AIDS Read, V16, P664

[9] Platelets may affect detection and quantitation of HIV RNA in plasma samples with low viral loads [J].

Sabino, EC ;

Gaburo, N ;

Leite, JP ;

Viecili, M ;

Rosenfeld, LG .

JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2004, 37 (03) :1432-1433

[10] Discordance between viral loads determined by Roche COBAS AMPLICOR human immunodeficiency virus type 1 Monitor (version 1.5) standard and ultrasensitive assays caused by freezing patient plasma in centrifuged Becton-Dickinson vacutainer brand plasma preparation tubes [J].

Salimnia, H ;

Moore, EC ;

Crane, LR ;

MacArthur, RD ;

Fairfax, MR .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (09) :4635-4639

← 1 2 →