Activation of PPARγ inhibits cell growth and induces apoptosis in human gastric cancer cells

被引：237

作者：

Takahashi, N

Okumura, T ^{[1
]}

Motomura, L

Fujimoto, Y

Kawabata, I

Kohgo, Y

机构：

[1] Asahikawa Med Coll, Dept Internal Med 3, Asahikawa, Hokkaido 0788510, Japan

[2] Asahikawa Med Coll, Dept Lab Med, Asahikawa, Hokkaido 0788510, Japan

来源：

FEBS LETTERS | 1999年 / 455卷 / 1-2期

关键词：

peroxisome proliferator-activated receptor gamma; growth inhibition; apoptosis; gastric cancer;

D O I：

10.1016/S0014-5793(99)00871-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) and the role of PPAR gamma in cell growth in human gastric cancer cells. Reverse transcription-polymerase chain reaction, Northern blot and Western blot analyses showed that a human gastric cancer cell line, MKN45, expressed PPAR gamma mRNA and protein. Luciferase assay in MKN45 cells showed that troglitazone, a selective ligand for PPAR gamma, transactivated the transcription of a peroxisome proliferator response element-driven promoter. Troglitazone or pioglitazone, selective ligands for PPAR gamma, inhibited the growth of MKN45 cells in a dose-dependent manner. Co-incubation of MKN45 cells with troglitazone induced DNA ladder formation. These results suggest that human gastric cancer cells express PPAR gamma and that activation of PPAR gamma inhibits cell growth and induces apoptosis in gastric cancer cells. (C) 1999 Federation of European Biochemical Societies.

引用

页码：135 / 139

页数：5

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