Microbial community responses to 17 years of altered precipitation are seasonally dependent and coupled to co-varying effects of water content on vegetation and soil C

被引:68
作者
Sorensen, Patrick O. [1 ]
Germino, Matthew J. [2 ]
Feris, Kevin P. [1 ]
机构
[1] Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA
[2] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID USA
基金
美国国家科学基金会;
关键词
Climate; Precipitation-timing; Artemisia tridentata; Cold desert; Sagebrush steppe; Carbon storage; Microbial community composition; FRAGMENT-LENGTH-POLYMORPHISMS; GREAT-BASIN; DIVERSITY; CARBON; ECOSYSTEM; RAINFALL; NITROGEN; PULSES; DYNAMICS; DEPTH;
D O I
10.1016/j.soilbio.2013.04.014
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Precipitation amount and seasonal timing determine the duration and distribution of water available for plant and microbial activity in the cold desert sagebrush steppe. In this study, we sought to determine if a sustained shift in the amount and timing of precipitation would affect soil microbial diversity, community composition, and soil carbon (C) storage. Field plots were irrigated (+200 mm) during the dormant or growing-season for 17 years. Microbial community responses were assessed over the course of a year at two depths (15-20 cm, 95-100 cm) by terminal restriction fragment length polymorphism (T-RFLP), along with co-occurring changes in plant cover and edaphic properties. Bacterial richness, Shannon Weaver diversity, and composition in shallow soils (15-20 cm) as well as evenness in deep soils (95-100 cm) differed across irrigation treatments during July. Irrigation timing affected fungal community diversity and community composition during the dormant season and most strongly in deep soils (95-100 cm). Dormant-season irrigation increased the ratio of shrubs to forbs and reduced soil C in shallow soils by 16% relative to ambient conditions. It is unclear whether or not soil C will continue to decline with continued treatment application or if microbial adaptation could mitigate sustained soil C losses. Future changes in precipitation timing will affect soil microbes in a seasonally dependent manner and be coupled to co-varying effects of water content on vegetation and soil C. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:155 / 163
页数:9
相关论文
共 49 条
[1]   A Shift in Seasonal Rainfall Reduces Soil Organic Carbon Storage in a Cold Desert [J].
Aanderud, Zachary T. ;
Richards, James H. ;
Svejcar, Tony ;
James, Jeremy J. .
ECOSYSTEMS, 2010, 13 (05) :673-682
[2]   Statistical methods for characterizing diversity of microbial communities by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes [J].
Abdo, Z ;
Schüette, UME ;
Bent, SJ ;
Williams, CJ ;
Forney, LJ ;
Joyce, P .
ENVIRONMENTAL MICROBIOLOGY, 2006, 8 (05) :929-938
[3]  
Anderson J., 2002, PROTECTIVE CAP BIOBA
[4]  
Anderson JE, 2001, ECOL MONOGR, V71, P531, DOI 10.1890/0012-9615(2001)071[0531:LSCIPS]2.0.CO
[5]  
2
[6]   Water pulses and biogeochemical cycles in arid and semiarid ecosystems [J].
Austin, AT ;
Yahdjian, L ;
Stark, JM ;
Belnap, J ;
Porporato, A ;
Norton, U ;
Ravetta, DA ;
Schaeffer, SM .
OECOLOGIA, 2004, 141 (02) :221-235
[7]   The tragedy of the uncommon: understanding limitations in the analysis of microbial diversity [J].
Bent, Stephen J. ;
Forney, Larry J. .
ISME JOURNAL, 2008, 2 (07) :689-695
[8]   Interpreting ecological diversity indices applied to terminal restriction fragment length polymorphism data: Insights from simulated microbial communities [J].
Blackwood, Christopher B. ;
Hudleston, Deborah ;
Zak, Donald R. ;
Buyer, Jeffrey S. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (16) :5276-5283
[9]  
Brooks PD, 1996, BIOGEOCHEMISTRY, V32, P93, DOI 10.1007/BF00000354
[10]   Winter production of CO2 and N2O from Alpine tundra: Environmental controls and relationship to inter-system C and N fluxes [J].
Brooks, PD ;
Schmidt, SK ;
Williams, MW .
OECOLOGIA, 1997, 110 (03) :403-413