Chemical reporters for biological discovery

被引：293

作者：

Grammel, Markus ^{[1
]}

Hang, Howard C. ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Chem Biol & Microbial Pathogenesis, New York, NY 10021 USA

来源：

NATURE CHEMICAL BIOLOGY | 2013年 / 9卷 / 08期

基金：

美国国家卫生研究院;

关键词：

NEWLY SYNTHESIZED PROTEINS; GLCNAC-MODIFIED PROTEINS; IN-VIVO; GENETIC-CODE; GLYCOSYLATION; DYNAMICS; ANALOG; DNA; METHYLTRANSFERASE; PALMITOYLATION;

D O I：

10.1038/nchembio.1296

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.

引用

页码：475 / 484

页数：10

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