High-yield production of a low-temperature-active polygalacturonase for papaya juice clarification

被引：75

作者：

Tu, Tao ^{[1
]}

Meng, Kun ^{[1
]}

Bai, Yingguo ^{[1
]}

Shi, Pengjun ^{[1
]}

Luo, Huiying ^{[1
]}

Wang, Yaru ^{[1
]}

Yang, Peilong ^{[1
]}

Zhang, Yuhong ^{[2
]}

Zhang, Wei ^{[2
]}

Yao, Bin ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Feed Res Inst, Key Lab Feed Biotechnol, Minist Agr, Beijing 100081, Peoples R China

[2] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

来源：

FOOD CHEMISTRY | 2013年 / 141卷 / 03期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Achaetomium sp Xz8; Endo-polygalacturonase; Low-temperature-active; High specific activity; Juice clarification; THERMOSTABLE POLYGALACTURONASE; ENDO-POLYGALACTURONASE; PURIFICATION; EXPRESSION; ENDOPOLYGALACTURONASE; OPTIMIZATION; PROTEINS; ENZYMES; PECTIN; TIME;

D O I：

10.1016/j.foodchem.2013.05.132

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A novel endo-polygalacturonase (endo-PG I) from Achaetomium sp. Xz8 was identified, overexpressed in Pichia pastoris, and characterized in this report. Recombinant endo-PG I is distinguished from other enzyme counterparts by its high activity towards polygalacturonic acid (49,934 U/ml) and high yield in the 15-1 fermentor (2.13 g/l). It exhibits optimal activity at 45 C and remained active over a broad temperature range of 0-80 degrees C. Distinct from most fungal polygalacturonases that have acidic pH optima, endo-PG I is optimally active at pH 6, similar to the pH of fresh papaya juice (5.7). Endo-PG I alone reduced the viscosity of papaya juice by 17.6%, and increased its transmittance by 59.1%. When combined with a commercial pectin methylesterase, it showed much higher efficiency with a synergy degree of more than 1.25. All these favourable enzymatic properties make endo-PG I attractive for potential applications in the juice industry. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：2974 / 2981

页数：8

共 30 条

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[2]

Heterologous protein expression in the methylotrophic yeast Pichia pastoris [J].