Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering

被引:92
作者
Guedon, E [1 ]
Desvaux, M [1 ]
Petitdemange, H [1 ]
机构
[1] Univ Henri Poincare, Fac Sci, Lab Biochim Bacteries Gram Positif, F-54506 Vandoeuvre Les Nancy, France
关键词
D O I
10.1128/AEM.68.1.53-58.2002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cellulolytic clostridia have evolved to catabolize lignocellulosic materials at a seasonal biorhythm, so their biotechnological exploitation requires genetic improvements. As high carbon flux leads to pyruvate accumulation, which is responsible for the cessation of growth of Clostridium cellulolyticum, this accumulation is decreased by heterologous expression of pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis. In comparison with that of the wild strain, growth of the recombinant strain at the same specific rate but for 145 h instead of 80 h led to a 150% increase in cellulose consumption and a 180% increase in cell dry weight. The fermentation pattern was shifted significantly: lactate production decreased by 48%, whereas the concentrations of acetate and ethanol increased by 93 and 53%, respectively. This study demonstrates that the fermentation of cellulose, the most abundant and renewable polymer on earth, can be greatly improved by using genetically engineered C. cellulolyticum.
引用
收藏
页码:53 / 58
页数:6
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