Improved separation of vitamin B complex and folic acid using some new solvent systems and impregnated TLC

TLC separation of vitamins of the 'B' complex group and folic acid has been achieved on plates impregnated with different transition metal ions. The metal ions used were Mn++, Fe++, Co++ Ni++, Cu++, Zn++ and Hg++. hR(f) values for all the vitamins, using six new solvent systems,worked out for the purpose, for each of the four different concentrations of each metal ion have been reported. The results have been discussed for each metal ion compared and the best conditions of separation have been identified.