The isolation and characterization of polysaccharides from longan pulp

被引：190

作者：

Yang, Cuixian ^{[1
]}

He, Ning ^{[1
]}

Ling, Xueping ^{[1
]}

Ye, Meiling ^{[1
]}

Zhang, Chenxiao ^{[1
]}

Shao, Wenyao ^{[1
]}

Yao, Chuanyi ^{[1
]}

Wang, Zhiyuan ^{[1
]}

Li, Qingbiao ^{[1
]}

机构：

[1] Xiamen Univ, Dept Chem & Biochem Engn, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen 361005, Peoples R China

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2008年 / 63卷 / 01期

关键词：

longan; polysaccharide; isolation; characterization;

D O I：

10.1016/j.seppur.2008.05.004

中图分类号：

TQ [化学工业];

学科分类号：

0817 [化学工程与技术];

摘要：

Crude water-soluble longan polysaccharides (LPSs) were extracted from longan pulp using hot water assisted by microwave pretreatment and ethanol precipitation. Neutral and acidic polysaccharides were separated by DEAE-cellulose anion-exchange chromatography. High performance gel permeation chromatography (HPGPC) analysis showed that the average molecular weight (M-w) of neutral polysaccharide (LPS-N) was approximately 13.8 kDa, and those of two acidic polysaccharicles (LPS-A1 and LPS-A2) were approximately 1382 and 571 kDa, respectively. Structural properties and compositions of these three LPS were examined by FTIR and HPLC. It was hypothesized that LPS belong to P-type acidic heteropolysaccharides with pyran group, among which LPS-N was composed of xylose and glucose at the molar ratio of 1:1.9; LPS-A1 consisted of rhamnose, xylose, arabinose and galactose at the molar ratio of 1:1.64:4.33:2.28; and LPS-A2 comprised only rhamnose. The total uronic acid content of LPS-A1 and LPS-A2 were 6% and 19%, respectively. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：226 / 230

页数：5

共 29 条

[1]

DETERMINATION OF THE MOLECULAR-WEIGHT OF CLINICAL DEXTRAN BY GEL-PERMEATION CHROMATOGRAPHY ON TSK-PW-TYPE COLUMNS [J].