Detection of Ag+ ions and cysteine based on chelation actions between Ag+ ions and guanine bases

被引：23

作者：

Chen, Xia ^{[1
,2
]}

Chen, Yinran ^{[1
,2
]}

Zhou, Xiaodong ^{[1
,2
]}

Hu, Jiming ^{[1
,2
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Key Lab Analyt Chem Biol & Med, Minist Educ, Wuhan 430072, Hubei, Peoples R China

[2] Wuhan Univ, Coll Chem & Mol Sci, Inst Analyt Biomed, Wuhan 430072, Hubei, Peoples R China

来源：

TALANTA | 2013年 / 107卷

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

Fluorescence; Biosensor; Ag+ ions; Cysteine; Graphene oxide; ATOMIC-ABSORPTION-SPECTROMETRY; WALLED CARBON NANOTUBES; SELECTIVE ELECTRODE; MULTIPLEX DETECTION; SENSING PLATFORM; WATER SAMPLES; LOGIC GATE; SILVER; DNA; DESIGN;

D O I：

10.1016/j.talanta.2013.01.025

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A selective and sensitive fluorescence biosensor for Ag+ ions and cysteine (Cys) was developed based on the chelation actions between Ag+ ions and guanine bases of G-rich fluorogenic oligonucleotide (FAM-ssDNA) and the different electrostatic affinity between FAM-ssDNA and graphene oxide (GO). FAM-ssDNA adsorbed onto the surface of GO through pi-pi stacking interaction between the ring structure in the nucleobases and the hexagonal cells of GO, and the fluorescence of the dye was quenched. In the presence of Ag+ ions, the random coil structure changed into a G-Ag+ architecture. As a result, the binding released FAM-ssDNA signal probe from the surface of GO, which disrupted the energy transfer from FAM-ssDNA to GO, recovering the fluorescence emission of FAM-ssDNA. On the other hand, because Cys was a strong Ag+ ions binder, it could deactivate the sensor fluorescence by rewrapping FAM-ssDNA around GO. In this way, these changes in fluorescence intensity allowed the selective detection of Ag+ ions and Cys. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：277 / 283

页数：7

共 36 条

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