Synthesis and characterization of oligodeoxyribonucleotides containing tandem base damage

被引：2

作者：

Bourdat, AG ^{[1
]}

Gasparutto, D ^{[1
]}

D'Ham, C ^{[1
]}

Cadet, J ^{[1
]}

机构：

[1] CEA Grenoble, Lab Les Acides Nucl, Serv Chim Inorgan & Biol, Dept Rech Fondamentale Mat Condensee, F-38054 Grenoble 9, France

来源：

NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 6-7期

关键词：

D O I：

10.1080/07328319908044713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Both N(2-deoxy-beta-D-erythro-pentofuranosyl)-formylamine (d beta F) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) were introduced in synthetic oligonucleotides at a vicinal position via the solid phase phosphoramidite method in order to investigate the biological and structural significance of such a tandem lesion. Further experiments aimed at determining the enzymatic repair by both E. coli endonuclease III (Endo III) and Fapy-glycosylase (Fpg) were carried out with these synthetic substrates.

引用

页码：1349 / 1350

页数：2

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