A structured chitosan-based platform for biomolecule attachment to solid surfaces: Application to DNA microarray preparation

被引：25

作者：

Consolandi, C

Severgnini, M

Castiglioni, B

Bordoni, R

Frosini, A

Battaglia, C

Bemardi, LR

De Bellis, G

机构：

[1] LITA, Consiglio Nazl Ric, Ist Tecnol Biomed, I-20090 Segrate, MI, Italy

[2] Univ Milan, Dipartimento Sci & Tecnol Biomed, Milan, Italy

来源：

BIOCONJUGATE CHEMISTRY | 2006年 / 17卷 / 02期

关键词：

D O I：

10.1021/bc050285a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternative chemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach has been adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-mer and 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotide loading capacity, good availability to hybridization against targets, and effectiveness in enzyme-mediated single nucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with low background. Universal arrays have been prepared and extensively used with excellent results in different applications. The chitosan-treated surfaces were also evaluated for their performance in a gene expression experiment.

引用

页码：371 / 377

页数：7

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