Structural transitions of a twisted and stretched DNA molecule

被引：237

作者：

Léger, JF

Romano, G

Sarkar, A

Robert, J

Bourdieu, L

Chatenay, D

Marko, JF

机构：

[1] LDFC, UMR CNRS 7506, F-67000 Strasbourg, France

[2] Univ Strasbourg 1, Inst Phys, F-67000 Strasbourg, France

[3] Univ Illinois, Dept Phys, Chicago, IL 60607 USA

来源：

PHYSICAL REVIEW LETTERS | 1999年 / 83卷 / 05期

关键词：

D O I：

10.1103/PhysRevLett.83.1066

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We report results of a micromanipulation study of single double-helical DNA molecules at forces up to 150 pN. Depending on whether the DNA winding is allowed to relax, or held fixed, qualitatively different structural transitions are observed. By studying the transitions as a function of winding the different DNA structures underlying them are characterized; this allows us to report the first estimate of S-DNA helicity. A model is introduced to describe these transitions; in addition to B-DNA, we find that four DNA states are needed to describe the experiments.

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页码：1066 / 1069

页数：4

相关论文

共 9 条

[1] Stretched and overwound DNA forms a Pauling-like structure with exposed bases [J].

Allemand, JF ;

Bensimon, D ;

Lavery, R ;

Croquette, V .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (24) :14152-14157

[2] DNA: An extensible molecule [J].

Cluzel, P ;

Lebrun, A ;

Heller, C ;

Lavery, R ;

Viovy, JL ;

Chatenay, D ;

Caron, F .

SCIENCE, 1996, 271 (5250) :792-794

[3] WRITHING NUMBER OF A SPACE CURVE [J].

FULLER, FB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (04) :815-&

[4] Modelling extreme stretching of DNA [J].

Lebrun, A ;

Lavery, R .

NUCLEIC ACIDS RESEARCH, 1996, 24 (12) :2260-2267

[5] RecA binding to a single double-stranded DNA molecule: A possible role of DNA conformational fluctuations [J].

Leger, JF ;

Robert, J ;

Bourdieu, L ;

Chatenay, D ;

Marko, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12295-12299

[6] DNA under high tension: Overstretching, undertwisting, and relaxation dynamics [J].

Marko, JF .

PHYSICAL REVIEW E, 1998, 57 (02) :2134-2149

[7] Overstretching B-DNA: The elastic response of individual double-stranded and single-stranded DNA molecules [J].

Smith, SB ;

Cui, YJ ;

Bustamante, C .

SCIENCE, 1996, 271 (5250) :795-799

[8] Behavior of supercoiled DNA [J].

Strick, TR ;

Allemand, JF ;

Bensimon, D ;

Croquette, V .

BIOPHYSICAL JOURNAL, 1998, 74 (04) :2016-2028

[9] The elasticity of a single supercoiled DNA molecule [J].

Strick, TR ;

Allemand, JF ;

Bensimon, D ;

Bensimon, A ;

Croquette, V .

SCIENCE, 1996, 271 (5257) :1835-1837