Protective effect of chitin oligosaccharides against lipopolysaccharide-induced inflammatory response in BV-2 microglia

被引：44

作者：

Thanh-Sang Vo ^{[1
]}

Dai-Hung Ngo ^{[1
]}

Quang Van Ta ^{[1
]}

Wijesekara, Isuru ^{[1
]}

Kong, Chang-Suk ^{[2
]}

Kim, Se-Kwon ^{[1
,3
]}

机构：

[1] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

[2] Silla Univ, Coll Med & Life Sci, Dept Food & Nutr, Pusan 617736, South Korea

[3] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea

来源：

CELLULAR IMMUNOLOGY | 2012年 / 277卷 / 1-2期

关键词：

Chitin oligosaccharides; Microglia; Lipopolysaccharide; Nuclear factor-kappa B; Cyclooxygenase-2; NF-KAPPA-B; ACTIVATED PROTEIN-KINASE; PARKINSONS-DISEASE; NEURODEGENERATIVE DISEASES; CYTOKINE EXPRESSION; THERAPEUTIC TARGET; REACTIVE OXYGEN; NITRIC-OXIDE; ANTIOXIDANT; INHIBITION;

D O I：

10.1016/j.cellimm.2012.06.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Chitin oligosaccharides (NA-COS) of two different molecular weight ranges (below 1 and 1-3 kDa) were examined for their capabilities against lipopolysaccharide-induced inflammatory responses in BV-2 murine It was found that NA-COS reduced the level of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production by suppressing the expression of NO synthase (iNOS) and cyclooxygenase (COX)-2 without significant cytotoxicity. Furthermore, the inhibitory effects of NA-COS on generation of interleukin (IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha were determined. Notably, NA-COS exerted anti-inflammatory activities via blocking degradation of inhibitor of kappaB-alpha (I kappa B-alpha), translocation of nuclear factor (NF)-kappa B, and phosphorylation of mitogen-activated protein kinases (MAPKs) in a dose-dependent manner. These findings provide mechanistic insights into the anti-inflammatory and neuroprotective actions of NA-COS in BV-2 microglia. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：14 / 21

页数：8

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